Human IgA(Immunoglobulin A) ELISA Kit
中文名称:人免疫球蛋白A(IgA)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IgA抗体包被于酶标板上,实验时样品(或标准品)中的人IgA会与包被抗体结合。后依次加入生物素化的抗人IgA抗体和辣根过氧化物酶标记的亲和素,抗人IgA抗体与结合在包被抗体上的人IgA结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IgA浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IgA的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IgA,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
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Q1:检测小鼠血清里的sIgA,会检测到IgA吗?血清样本中的IgA会干扰sIgA的检测吗?
样本中的IgA是可以检测到的,这主要是基于IgA和sIgA结构的重叠,会存在一定的交叉反应。
小鼠和人类的IgA生物学在几个方面有所不同。在人血清中,IgA主要以单体形式存在,而在小鼠血清中,IgA主要以聚合形式存在。此外,人类IgA可分为密切相关的IgAl和IgA2亚类,其中IgA2较不易被蛋白水解降解。在血清中,IgAl亚类占主导地位,而在分泌物中发现的主要亚型是IgA2,尽管IgAl和IgA2都可以检测到sIgA。