Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit
中文名称：人胰岛素样生长因子1(IGF-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 ng/mL
- 灵敏度： 0.94 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IGF-1抗体包被于酶标板上，实验时样品（或标准品）中的人IGF-1会与包被抗体结合。后依次加入生物素化的抗人IGF-1抗体和辣根过氧化物酶标记的亲和素，抗人IGF-1抗体与结合在包被抗体上的人IGF-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IGF-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IGF-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 ng/mL |
| 灵敏度 | 0.94 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IGF-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Neurons enhance blood–brain barrier function via upregulating claudin-5 and VE-cadherin expression due to glial cell line-derived neurotrophic factor secretion
IF: 6.400 -
Influence of CReatine Supplementation on mUScle Mass and Strength After Stroke (ICaRUS Stroke Trial): A Randomized Controlled Trial
IF: 4.800 -
Exploring Vitamin D Deficiency and IGF Axis Dynamics in Colorectal Adenomas
IF: 3.900 -
Clinical Characteristics, and Prevalence of Hepatic and Bone Mineral Density Abnormalities in Patients with Sheehan Syndrome: Data from a Tertiary Care Centre
IF: 3.700Journal： Endocrine Practice (2024)Reactivity：HumanSample Type：serum -
Long-term stability of frozen platelet-rich plasma under??80?°C storage condition
IF: 3.400Journal： Regenerative Therapy (2024)Reactivity：HumanSample Type：Platelet rich plasma (PRP) -
Comparison of growth factor levels in injectable platelet-rich fibrin obtained from healthy individuals and patients with chronic periodontitis: a pilot study
IF: 2.600 -
MiR-19b-3p inhibits cell viability and proliferation and promotes apoptosis by targeting IGF1 in KGN cells
IF: 2.000Journal： GYNECOLOGICAL ENDOCRINOLOGY (2024)Reactivity：HumanSample Type：Follicular fluid -
Insulin-Like Growth Factor-1 and Insulin-Like Growth Factor Binding Protein-3 - Biomarkers for Skeletal Maturity Assessment in Class II Malocclusion
IF: 0.400Journal： Pesquisa Brasileira em Odontopediatria e Clinica Integrada (2024)Reactivity：HumanSample Type：serum -
LncCCLM inhibits lymphatic metastasis of cervical cancer by promoting STAU1-mediated IGF-1 mRNA degradation
IF: 8.679 -
Mechanical Stress-Induced IGF-1 Facilitates col-I and col-III Synthesis via the IGF-1R/AKT/mTORC1 Signaling Pathway
IF: 5.443
实验操作视频
