Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Expansion of GARP-Expressing CD4+CD25?FoxP3+ T Cells and SATB1 Association with Activation and Coagulation in Immune Compromised HIV-1-Infected Individuals in South Africa
IF: 4.327 -
Occupational exposure of rural workers to pesticides in a vegetable-producing region in Brazil
IF: 4.223Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021)PMID：33469792Reactivity：HumanSample Type：Plasma -
Biochemical investigation of human exposure to aflatoxin M1 and its association with risk factors of diabetes mellitus
IF: 4.223Journal： ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021)PMID：34216342Reactivity：HumanSample Type：serum -
A functional polymorphism at the miR?491?5p binding site in the 3'?untranslated region of the MMP?9 gene increases the risk of developing ventilator?associated pneumonia
IF: 4.101Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2021)PMID：34664683Reactivity：HumanSample Type：peripheral blood -
The effects of Mediterranean diet on hepatic steatosis, oxidative stress, and inflammation in adolescents with non-alcoholic fatty liver disease: A randomized controlled trial
IF: 4.000 -
Silencing of UCA1 Protects Against MPP+-Induced Cytotoxicity in SK-N-SH Cells via Modulating KCTD20 Expression by Sponging miR-423-5p
IF: 3.996Journal： NEUROCHEMICAL RESEARCH (2021)PMID：33464446Reactivity：HumanSample Type：Cell culture supernatant -
A simple lectin-based biochip might display the potential clinical value of glycomics in patients with spontaneous intracerebral hemorrhage
IF: 3.932 -
Combination of oncolytic adenovirus ZD55 harboring TRAIL-IETD-MnSOD and cytokine-induced killer cells against lung cancer
IF: 3.932Journal： Annals of Translational Medicine (2021)PMID：34790733Reactivity：Human,MouseSample Type：serum -
Establishing SW1353 Chondrocytes as a Cellular Model of Chondrolysis
IF: 3.817 -
A pathway phenotype linking metabolic, immune, oxidative, and opioid pathways with comorbid depression, atherosclerosis, and unstable angina
IF: 3.790
实验操作视频
