Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Role of IL-22 in persistent allergic airway diseases caused by house dust mite: a pilot study
IF: 3.317Journal： BMC Pulmonary Medicine (2021)PMID：33478443Reactivity：HumanSample Type：Serum,Nasal Lavage Fluid -
Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens
IF: 3.317Journal： BMC Pulmonary Medicine (2021)PMID：34930201Reactivity：HumanSample Type：serum, nasal lavage -
Evaluation of IL-10, IFN-γ, and thiol–disulfide homeostasis in patients with drug-resistant epilepsy
IF: 3.307 -
Carvedilol activates nuclear factor E2-related factor 2/ antioxidant response element pathway to inhibit oxidative stress and apoptosis of retinal pigment epithelial cells induced by high glucose
IF: 3.269 -
Circ_USP36 Silencing Attenuates Oxidized Low-Density Lipoprotein-Induced Dysfunction in Endothelial Cells in Atherosclerosis Through Mediating miR-197-3p/ROBO1 Axis
IF: 3.105 -
Identification of key genes and crucial pathways for major depressive disorder using peripheral blood samples and chronic unpredictable mild stress rat models
IF: 2.984 -
Transforming growth factor?β1 functions as a competitive endogenous RNA that ameliorates intracranial hemorrhage injury by sponging microRNA?93?5p
IF: 2.952Journal： Molecular Medicine Reports (2021)PMID：33955515Reactivity：HumanSample Type：Cell culture supernatant -
Carotid intima-media thickness and serum proinflammatory cytokine levels in rosacea patients without cardiovascular risk factors
IF: 2.851 -
XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway
IF: 2.847 -
Biochemical Mapping of the Inflamed Human Dental Pulp
IF: 2.679Journal： Applied Sciences-Basel (2021)Reactivity：HumanSample Type：dental pulp lysates
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