Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function
IF: 5.223Journal： CLINICAL SCIENCE (2020)PMID：32219341Reactivity：HumanSample Type：Cell culture supernatant -
Fontan protein-losing enteropathy is associated with advanced liver disease and a proinflammatory intestinal and systemic state
IF: 5.175 -
CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation
IF: 4.886Journal： JOURNAL OF IMMUNOLOGY (2020)PMID：32303555Reactivity：HumanSample Type：Cell culture supernatant -
In situ self-assembly of Au-antimiR-155 nanocomplexes mediates TLR3-dependent apoptosis in hepatocellular carcinoma cells.
IF: 4.831 -
Short-Term Physiological Effects of a Very Low-Calorie Ketogenic Diet: Effects on Adiponectin Levels and Inflammatory States
IF: 4.556Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)PMID：32370212Reactivity：HumanSample Type：serum -
LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD
IF: 4.545Journal： BIOMEDICINE & PHARMACOTHERAPY (2020)PMID：32145584Reactivity：HumanSample Type：Cell culture supernatant -
Long non-coding RNA NNT-AS1 regulates proliferation, apoptosis, inflammation and airway remodeling of chronic obstructive pulmonary disease via targeting miR-582-5p/FBXO11 axis
IF: 4.545Journal： BIOMEDICINE & PHARMACOTHERAPY (2020)PMID：32768929Reactivity：HumanSample Type：Cell culture supernatant -
S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes
IF: 4.352Journal： BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2020)PMID：32681864Reactivity：HumanSample Type：Serum -
NRF2 -617 C/A Polymorphism Impacts Proinflammatory Cytokine Levels, Survival, and Transplant-Related Mortality After Hematopoietic Stem Cell Transplantation in Adult Patients Receiving Busulfan-Based Conditioning Regimens
IF: 4.225 -
Inhibition of TLR2/TLR4 alleviates the Neisseria gonorrhoeae infection damage in human endometrial epithelial cells via Nrf2 and NF-Kβsignaling
IF: 4.018Journal： JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2020)PMID：32950783Reactivity：HumanSample Type：Cell culture supernatant
实验操作视频
