Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits
IF: 3.998Journal： Scientific Reports (2020)PMID：32669704Reactivity：HumanSample Type：Cell culture supernatant -
HDAC1 promotes artery injury through activation of VAV3 by binding to miR-182-5p in atherosclerotic mice model
IF: 3.968Journal： CELLULAR SIGNALLING (2020)PMID：33221374Reactivity：HumanSample Type：Cell culture supernatant -
Thymosin β10 promotes tumor-associated macrophages M2 conversion and proliferation via the PI3K/Akt pathway in lung adenocarcinoma
IF: 3.924 -
Construction of an exposure-pathway-phenotype in children with depression due to transfusion-dependent thalassemia: Results of (un)supervised machine learning.
IF: 3.892 -
Induction of CD4+CD25+ Regulatory T Cells from In Vitro Grown Human Mononuclear Cells by Sparteine Sulfate and Harpagoside
IF: 3.796 -
Chronic fatigue and fibromyalgia symptoms are key components of deficit schizophrenia and are strongly associated with activated immune-inflammatory pathways
IF: 3.759 -
Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways
IF: 3.690Journal： JOURNAL OF ETHNOPHARMACOLOGY (2020)PMID：32497675Reactivity：HumanSample Type：Serum,Cell culture supernatant -
Nec-1 Attenuates Neurotoxicity Induced by Titanium Dioxide Nanomaterials on Sh-Sy5y Cells Through RIP1
IF: 3.578 -
iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin–angiotensin system-mediated congenital ureteropelvic junction obstruction
IF: 3.383Journal： EXPERIMENTAL CELL RESEARCH (2020)PMID：32416091Reactivity：HumanSample Type：Serum,Cell sample -
A Novel 7-Days Prolonged Dietary Deprivation Regimen Improves ALT and UA After 3–6 Months Refeeding, Indicating Therapeutic Potential
IF: 3.365
实验操作视频
