Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
MiRNA505/NET1 Axis Acts as a CD8+ T-TIL Regulator in Non-Small Cell Lung Cancer
IF: 3.337Journal： OncoTargets and Therapy (2020)PMID：33061457Reactivity：HumanSample Type：Cell culture supernatant -
Human cytomegalovirus infection is correlated with atherosclerotic plaque vulnerability in carotid artery
IF: 3.258Journal： JOURNAL OF GENE MEDICINE (2020)DOI：10.1002/jgm.3236PMID：32468600Reactivity：HumanSample Type：Serum -
Trace element, immune and opioid biomarkers of unstable angina, increased atherogenicity and insulin resistance: Results of machine learning
IF: 3.245Journal： JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2020)PMID：33338984Reactivity：HumanSample Type：serum -
Cytokine profile during pregnancy predicts relapses during pregnancy and postpartum in multiple sclerosis
IF: 3.115Journal： JOURNAL OF THE NEUROLOGICAL SCIENCES (2020)PMID：32278167Reactivity：HumanSample Type：serum -
The Endogenous Opioid System in Schizophrenia and Treatment Resistant Schizophrenia: Increased Plasma Endomorphin 2, and κ and μ Opioid Receptors Are Associated with Interleukin-6
IF: 3.110 -
MiR-942-5p alleviates septic acute kidney injury by targeting FOXO3
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572890Reactivity：HumanSample Type：Cell culture supernatant -
The Neuroimmune and Neurotoxic Fingerprint of Major Neurocognitive Psychosis or Deficit Schizophrenia: a Supervised Machine Learning Study
IF: 2.992 -
Major Depression in Children with Transfusion-Dependent Thalassemia Is Strongly Associated with the Combined Effects of Blood Transfusion Rate, Iron Overload, and Increased Pro-inflammatory Cytokines
IF: 2.992 -
A novel human anti-TIGIT monoclonal antibody with excellent function in eliciting NK cell-mediated antitumor immunity
IF: 2.985Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)PMID：33341068Reactivity：HumanSample Type：Cell culture supernatant -
The Value of Extracellular Cold-Inducible RNA-Binding Protein (eCIRP) in Predicting the Severity and Prognosis of Patients After Cardiac Arrest: A Preliminary Observational Study
IF: 2.960
实验操作视频
