Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Chronic fatigue syndrome and fibromyalgia-like symptoms are an integral component of the phenome of schizophrenia: neuro-immune and opioid system correlates
IF: 2.726 -
Early HIV infection is associated with reduced proportions of gamma delta T subsets as well as high creatinine and urea levels
IF: 2.717 -
XIST silencing alleviated inflammation and mesangial cells proliferation in diabetic nephropathy by sponging miR-485
IF: 2.575Journal： ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020)PMID：32669002Reactivity：HumanSample Type：Serum -
Obstructive sleep apnea in children with hypothalamic obesity: Evaluation of possible related factors
IF: 2.534 -
Long Noncoding RNA PMS2L2 Downregulates miR-24 through Methylation to Suppress Cell Apoptosis in Ulcerative Colitis
IF: 2.493 -
The clinical value of interleukins-8, -10, and -17 in idiopathic granulomatous mastitis
IF: 2.394 -
A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions
IF: 2.352Journal： IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY (2020)PMID：32605504Reactivity：HumanSample Type：Serum -
Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial
IF: 2.276 -
Heme is involved in the systemic inflammatory response following radiofrequency ablation of hepatic hemangiomas
IF: 2.251Journal： EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY (2020)PMID：31851092Reactivity：HumanSample Type：serum -
Intermittent hypoxia induces tumor immune escape in murine S180 solid tumors via the upregulation of TGF-β1 in mice
IF: 2.162
实验操作视频
