Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Auto-reactivity against gut bacterial peptides in patients with late-onset diabetes
IF: 2.125 -
Penehyclidine hydrochloride alleviates lipopolysaccharide?induced acute respiratory distress syndrome in cells via regulating autophagy?related pathway
IF: 2.100Journal： Molecular Medicine Reports (2020)PMID：33300058Reactivity：HumanSample Type：Cell culture supernatant -
Clinical characteristics of 34 COVID-19 patients admitted to intensive care unit in Hangzhou, China
IF: 2.082Journal： Journal of Zhejiang University-SCIENCE B (2020)Reactivity：HumanSample Type：Plasma -
Investigation of DNA damage, oxidative stress, and inflammation in synthetic cannabinoid users
IF: 2.067 -
Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1
IF: 1.931Journal： ARCHIVES OF ORAL BIOLOGY (2020)PMID：32805637Reactivity：HumanSample Type：Cell culture supernatant -
Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells
IF: 1.918Journal： MEDICAL SCIENCE MONITOR (2020)PMID：32463805Reactivity：HumanSample Type：Cell culture supernatant -
Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments
IF: 1.904 -
Rhubarb-Aconite Decoction (RAD) Drug-Containing Serum Alleviated Endotoxin-Induced Oxidative Stress Injury and Inflammatory Response in Caco-2 Cells In Vitro
IF: 1.813Journal： Evidence-based Complementary and Alternative Medicine (2020)PMID：32714409Reactivity：HumanSample Type：Cell culture supernatant -
Application of dexmedetomidine-assisted intravertebral anesthesia in hip replacement and its influence on T-lymphocyte subsets
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32742362Reactivity：HumanSample Type：serum -
Mycobacterium tuberculosis infection increases the number of osteoclasts and inhibits osteoclast apoptosis by regulating TNF?α?mediated osteoclast autophagy
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：32782497Reactivity：HumanSample Type：Cell culture supernatant
实验操作视频
