Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Increased expression of IFN?γ in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop
IF: 1.785Journal： Experimental and Therapeutic Medicine (2020)PMID：33335575Reactivity：HumanSample Type：Tissue homogenate -
Regulatory role and mechanism of the inhibition of the Mcl-1 pathway during apoptosis and polarization of H37Rv-infected macrophages
IF: 1.552 -
Correlation Between Single Nucleotide Polymorphisms at the 3′-UTR of the NFKB1 Gene and Acute Kidney Injury in Sepsis
IF: 1.378Journal： Genetic Testing and Molecular Biomarkers (2020)PMID：32315555Reactivity：HumanSample Type：Cell sample -
Effect of laparoscopic sleeve gastrectomy on renal function in obese patients
IF: 1.355 -
HMGB-1, TLR4, IL-1R1, TNF-α, and IL-1β: novel epilepsy markers?
IF: 1.276 -
Effects of Edaravone on Neurological Function and Tumor Necrosis Factor Alpha and Interleukin 8 Levels in Patients with Cerebral Infarction
IF: 1.182 -
Green coffee extract modifies body weight, serum lipids and TNF-α in high-fat diet-induced obese rats
-
Molecular characterization, inducible expression and functional analysis of an IKKβ from the tropical sea cucumber Holothuria leucospilota
Journal： FISH & SHELLFISH IMMUNOLOGY (2020)PMID：32585358Reactivity：HumanSample Type：Cell culture supernatant -
Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD
IF: 14.971 -
Red Blood Cells-Derived Vesicles for Delivery of Lipophilic Drug Camptothecin
IF: 8.456
实验操作视频
