Human IL-10(Interleukin 10)ELISA Kit
中文名称:人白介素10(IL-10)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.94 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上,实验时样品(或标准品)中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素,抗人IL-10抗体与结合在包被抗体上的人IL-10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.94 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
IF: 3.743Journal: BIOMEDICINE & PHARMACOTHERAPY (2019)PMID:30844656Reactivity:HumanSample Type:Wound drainage fluid -
Eriodictyol inhibits survival and inflammatory responses and promotes apoptosis in rheumatoid arthritis fibroblast-like synoviocytes through AKT/FOXO1 signaling
IF: 3.448 -
Notopterygium incisum extract (NRE) rescues cognitive deficits in APP/PS1 Alzhneimer's disease mice by attenuating amyloid-beta, tau, and neuroinflammation pathology
IF: 3.414Journal: JOURNAL OF ETHNOPHARMACOLOGY (2019)PMID:31783135Reactivity:HumanSample Type:Cell culture supernatant -
Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-na?ve rheumatoid arthritis patients
IF: 3.361 -
DosR antigen Rv1737c induces activation of macrophages dependent on the TLR2 pathway
IF: 3.291 -
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals
IF: 3.239Journal: DIABETES RESEARCH AND CLINICAL PRACTICE (2019)PMID:31150725Reactivity:HumanSample Type:Serum -
Salidroside ameliorates endothelial inflammation and oxidative stress by regulating the AMPK/NF-κB/NLRP3 signaling pathway in AGEs-induced HUVECs
IF: 3.170 -
IL-16 regulates macrophage polarization as a target gene of mir-145-3p
IF: 3.064 -
Common variants of genes encoding TLR4 and TLR4 pathway members TIRAP and IRAK1 are effective on MCP1, IL6, IL1β, and TNFα levels in type 2 diabetes and insulin resistance
IF: 3.061 -
Effect of Diosmin Administration in Patients with Chronic Venous Disorders on Selected Factors Affecting Angiogenesis
IF: 3.060

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