Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles
IF: 2.581Journal： MICROBIAL PATHOGENESIS (2019)PMID：31493501Reactivity：HumanSample Type：Cell culture supernatant -
Serum profile of cytokines and their genetic variants in metabolic syndrome and healthy subjects: a comparative study
IF: 2.535 -
Study of Metal-Metal Interactions and Their Biomarkers Using an Intestinal Human Cell Line
IF: 2.431Journal： BIOLOGICAL TRACE ELEMENT RESEARCH (2019)PMID：31473895Reactivity：HumanSample Type：Cell lysate -
The role of serum and urinary biomarkers in the diagnosis of early diabetic nephropathy in patients with type 2 diabetes
IF: 2.353 -
Autoantibodies to a specific peptide epitope of human Hsp60 (ATVLA) with homology to Helicobacter pylori HspB in H.?pylori-infected patients
IF: 2.225 -
Nigella sativa Oil Mouth Rinse Improves Chemotherapy-Induced Oral Mucositis in Patients with Acute Myeloid Leukemia
IF: 2.197 -
Effect of Helicobacter pylori-eradication therapy on hepatic steatosis in patients with non-alcoholic fatty liver disease: a randomized–controlled pilot study
IF: 2.194 -
In vitro impact of bisphenol A on maturation and function of monocyte-derived dendritic cells in patients with primary Sj?gren’s syndrome
IF: 2.086 -
Serum irisin level in myocardial infarction patients with or without heart failure
IF: 2.041Journal： CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY (2019)PMID：30958967Reactivity：HumanSample Type：serum -
The effect of different dietary ratios of arginine, methionine, and lysine on the performance, carcass traits, and immune status of turkeys
IF: 2.027
实验操作视频
