Human IL-10(Interleukin 10)ELISA Kit
中文名称：人白介素10(IL-10)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上，实验时样品（或标准品）中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素，抗人IL-10抗体与结合在包被抗体上的人IL-10结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-10浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-10的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-10，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway
IF: 3.118Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2018)PMID：30476824Reactivity：HumanSample Type：Cell culture supernatant -
LncRNA MALAT1 promotes relapse of breast cancer patients with postoperative fever
IF: 3.061Journal： American Journal of Translational Research (2018)DOI：PMID:30416660PMID：30416660Reactivity：HumanSample Type：plasma -
Assessment of chemokine and cytokine signatures in patients with dengue infection: A hospital-based study in Kolkata, India
IF: 2.509 -
Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response
IF: 1.894 -
Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-κB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children
IF: 1.894 -
N?methyl pyrrolidone promotes ankle fracture healing by inhibiting inflammation via suppression of the mitogen?activated protein kinase signaling pathway
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：29545891Reactivity：HumanSample Type：peripheral blood -
Leukocyte depletion does not affect the in?vitro healing ability of platelet rich plasma
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：29556269Reactivity：HumanSample Type：Cell culture supernatant -
Anti?inflammatory effect of Resolvin D1 on LPS?treated MG?63 cells Corrigendum in /10.3892/etm.2019.8021
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30402165Reactivity：HumanSample Type：Serum -
MicroRNA-124 alleviates hyperoxia-induced inflammatory response in pulmonary epithelial cell by inhibiting TLR4/NF-κB/CCL2
IF: 1.396Journal： International Journal of Clinical and Experimental Pathology (2018)DOI：PMID:31938089PMID：31938089Reactivity：Human -
CD4+Foxp3+ T cells, interleukin-35 (IL-35) and IL-10 in systemic lupus erythematosus patients: Relation to disease activity
Journal： Egyptian Rheumatologist (2018)Reactivity：HumanSample Type：serum
实验操作视频
