Human IL-10(Interleukin 10)ELISA Kit
中文名称:人白介素10(IL-10)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.94 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-10抗体包被于酶标板上,实验时样品(或标准品)中的人IL-10会与包被抗体结合。后依次加入生物素化的抗人IL-10抗体和辣根过氧化物酶标记的亲和素,抗人IL-10抗体与结合在包被抗体上的人IL-10结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-10浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-10的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.94 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-10,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Efficacy of Fire-Needle Therapy in Improving Neurological Function Following Cerebral Infarction and Its Effect on Intestinal Flora Metabolites
IF: 2.3Journal: International Journal of General Medicine (2024)PMID:38333018Reactivity:HumanSample Type:serum -
The Therapeutic Role and Mechanism of Glabridin Under Aspergillus fumigatus Infection
IF: 2.3 -
Prognostic potency of plasma LRG1 measurement at multiple time points in acute ischemic stroke patients
IF: 2.2 -
Effect of Millet Polyphenol-Rich Extracts on Human Fecal Bacteria and Dextran Sulfate Sodium-Induced Human Intestinal Epithelial Cell Derangements
IF: 1.8 -
Anesthesia Medication’s Impacts on Inflammatory and Neuroendocrine Immune Response in Patients Undergoing Digestive Endoscopy
IF: 1.7Journal: Clinics and Practice (2024)Reactivity:HumanSample Type:serum -
IL-27 attenuated macrophage injury and inflammation induced by Mycobacterium tuberculosis by activating autophagy
IF: 1.5Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2024)Reactivity:Human -
Using 6-CIT, P300 encephalography, and pro-inflammation assessments for screening age-related cognitive decline and exploring associated risk factors in Egyptian elderly
IF: 1.3Journal: Egyptian Journal of Neurology Psychiatry and Neurosurgery (2024)Reactivity:HumanSample Type:serum -
The effect of pectointercostal fascial block on stress response in open heart surgery
IF: 1.2 -
A second-generation M1-polarized CAR macrophage with antitumor efficacy
IF: 30.5 -
Fusobacterium nucleatum aggravates rheumatoid arthritis through FadA-containing outer membrane vesicles
IF: 30.300

暂无文件,请在搜索框输入识别码查询相关文件

名称 | 状态 | 有效期至 | 操作 | |
---|---|---|---|---|
{{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注:请在有效期内下载相关文件,过期后页面内将不提供文件下载及预览!