Human IL-18(Interleukin 18) ELISA Kit
中文名称：人白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-18抗体包被于酶标板上，实验时样品（或标准品）中的人IL-18会与包被抗体结合。后依次加入生物素化的抗人IL-18抗体和辣根过氧化物酶标记的亲和素，抗人IL-18抗体与结合在包被抗体上的人IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
IL-18 serves as a main effector of CAF-derived METTL3 against immunosuppression of NSCLC via driving NF-κB pathway
IF: 3.7 -
Altered ventricular longitudinal strain in children with sickle cell disease: Role of TGF-β and IL-18
IF: 3.2 -
Cell surface GRP78: A potential therapeutic target for high glucose-induced endothelial injury
IF: 3.1 -
The effect of secukinumab treatment for psoriasis on serum cytokines and correlation with disease severity
IF: 2.2 -
Upregulation of HTRA1 mediated by the lncRNA NEAT1/miR-141-3p axis contributes to endometriosis development through activating NLRP3 inflammasome-mediated pyroptotic cell death and cellular inflammation
IF: 2.100Journal： IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2023)PMID：37017808Reactivity：HumanSample Type：Endometrial tissue -
Role and significance of SIRT1 in regulating the LPS-activated pyroptosis pathway in children with congenital hydronephrosis
IF: 0.8 -
Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11–Mediated IL18 in the Gut Epithelial Cells
IF: 8.797Journal： Cellular and Molecular Gastroenterology and Hepatology (2022)PMID：36436756Reactivity：HumanSample Type：human colon organoid -
Hypoxia-Induced HIF-1α Expression Promotes Neurogenic Bladder Fibrosis via EMT and Pyroptosis
IF: 7.666 -
N-3 polyunsaturated fatty acids protect esophageal epithelial cells from acid exposure
IF: 7.425 -
Autophagy promotes GSDME-mediated pyroptosis via intrinsic and extrinsic apoptotic pathways in cobalt chloride-induced hypoxia reoxygenation-acute kidney injury
IF: 7.129
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