Human IL-18(Interleukin 18) ELISA Kit
中文名称:人白介素18(IL-18)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 15.63-1000 pg/mL
- 灵敏度: 9.38 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-18抗体包被于酶标板上,实验时样品(或标准品)中的人IL-18会与包被抗体结合。后依次加入生物素化的抗人IL-18抗体和辣根过氧化物酶标记的亲和素,抗人IL-18抗体与结合在包被抗体上的人IL-18结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-18浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-18的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 15.63-1000 pg/mL |
灵敏度 | 9.38 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-18,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
CCN1/Integrin α5β1 Instigates Free Fatty Acid-Induced Hepatocyte Lipid Accumulation and Pyroptosis through NLRP3 Inflammasome Activation
IF: 6.706 -
Chlorogenic acid attenuates inflammation in LPS-induced Human gingival fibroblasts via CysLT1R/Nrf2/NLRP3 signaling
IF: 5.714 -
Acrylamide Induces Neurotoxicity in SH-SY5Y Cells via NLRP3-mediated Pyroptosis
IF: 5.682 -
Long Noncoding RNA MIAT Regulates Hyperosmotic Stress-Induced Corneal Epithelial Cell Injury via Inhibiting the Caspase-1-Dependent Pyroptosis and Apoptosis in Dry Eye Disease
IF: 4.631 -
Ceramide induces pyroptosis through TXNIP/NLRP3/GSDMD pathway in HUVECs
IF: 2.813 -
Histone deacetylase 2 regulates ULK1 mediated pyroptosis during acute liver failure by the K68 acetylation site
IF: 8.469Journal: Cell Death & Disease (2021)PMID:33431796Reactivity:HumanSample Type:Serum,Cell culture supernatant -
Monocarboxylate Transporter 4 Triggered Cell Pyroptosis to Aggravate Intestinal Inflammation in Inflammatory Bowel Disease
IF: 7.561Journal: Frontiers in Immunology (2021)Reactivity:HumanSample Type:serum -
Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration
IF: 6.684 -
ESAT-6 impedes IL-18 mediated phagosome lysosome fusion via microRNA-30a upon Calcimycin treatment in mycobacteria infected macrophages
IF: 4.932 -
Moesin Is a Novel Biomarker of Endothelial Injury in Sepsis
IF: 4.818Journal: Journal of Immunology Research (2021)PMID:33628853Reactivity:HumanSample Type:Cell culture supernatant(HMECs)

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