Human IL-18(Interleukin 18) ELISA Kit
中文名称：人白介素18(IL-18)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-18抗体包被于酶标板上，实验时样品（或标准品）中的人IL-18会与包被抗体结合。后依次加入生物素化的抗人IL-18抗体和辣根过氧化物酶标记的亲和素，抗人IL-18抗体与结合在包被抗体上的人IL-18结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-18浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-18的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-18，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Comparison of Inflammation Biomarkers among Chronic Obstructive Pulmonary Disease Groups: A Cross Sectional Study
IF: 0.968Journal： Nigerian Journal of Clinical Practice (2021)Reactivity：HumanSample Type：serum -
Electrical stimulation inhibits Val-boroPro-induced pyroptosis in THP-1 macrophages via sirtuin3 activation to promote autophagy and inhibit ROS generation
IF: 4.831 -
MiR-1202 Exerts Neuroprotective Effects on OGD/R Induced Inflammation in HM Cell by Negatively Regulating Rab1a Involved in TLR4/NF-κB Signaling Pathway
IF: 3.038Journal： NEUROCHEMICAL RESEARCH (2020)PMID：32124161Reactivity：HumanSample Type：Cell culture medium -
Metformin alleviates high glucose-induced ER stress and inflammation by inhibiting the interaction between caveolin1 and AMPKα in rat astrocytes
IF: 2.985Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2020)PMID：33143871Reactivity：HumanSample Type：Cell culture supernatant -
miR-149-5p mitigates tumor necrosis factor-α-induced chondrocyte apoptosis by inhibiting TRADD
IF: 2.807 -
CD147 Aggravated Inflammatory Bowel Disease by Triggering NF-κB-Mediated Pyroptosis
IF: 2.276 -
Evaluation of alpha defensin, IL-1 receptor antagonist, and IL-18 levels in COVID-19 patients with macrophage activation syndrome and acute respiratory distress syndrome
IF: 2.021 -
Mechanistic Insight Into the Activation of the NLRP3 Inflammasome by Neisseria gonorrhoeae in Macrophages
IF: 4.716 -
Fatty acylCoA synthetase FadD13 regulates proinflammatory cytokine secretion dependent on the NF-κB signalling pathway by binding to eEF1A1
IF: 4.288Journal： CELLULAR MICROBIOLOGY (2019)PMID：31364251Reactivity：HumanSample Type：Cell culture supernatant -
The first cloned echinoderm tumor necrosis factor receptor from Holothuria leucospilota: Molecular characterization and functional analysis
IF: 3.298Journal： FISH & SHELLFISH IMMUNOLOGY (2019)PMID：31394160Reactivity：HumanSample Type：Cell culture supernatant
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