Human IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称:人白介素1β(IL-1β)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 7.81-500 pg/mL
- 灵敏度: 4.69 pg/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上,实验时样品(或标准品)中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素,抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,IL-1β浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-1β的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 7.81-500 pg/mL |
灵敏度 | 4.69 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-1β,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Vitexin Suppresses High-Glucose-upregulated Adhesion Molecule Expression in Endothelial Cells through Inhibiting NF-κB Signaling Pathway
IF: 3.7 -
TRIM47 promotes HDM-induced bronchial epithelial pyroptosis by regulating NEMO ubiquitination to activate NF-κB/NLRP3 signaling
IF: 3.3 -
Ginsenoside Rb3 alleviates the formation of osteoclasts induced by periodontal ligament fibroblasts in the periodontitis microenvironment through the STAT3 pathway
IF: 3.3 -
Clinical significance and underlying mechanism of long non-coding RNA SNHG12 in lower extremity deep venous thrombosis
IF: 3.1 -
Non-Surgical Periodontal Therapy’s Influence on Alpha-Synuclein and Inflammatory Marker Levels: A Pilot Study
IF: 3.0Journal: Journal of Clinical Medicine (2024)Reactivity:HumanSample Type:salivary,blood -
Long noncoding RNA NONHSAT122636.2 attenuates myocardial inflammation and apoptosis in myocarditis
IF: 2.9 -
Bleomycin induces endothelial cell pyroptosis and regulates fibrosis by activating the NLRP3/caspase-1/GSDMD pathway: a possible mechanism contributing to the sclerotherapy of venous malformations
IF: 2.9 -
Interaction between CD244 and SHP2 regulates inflammation in chronic obstructive pulmonary disease via targeting the MAPK/NF-κB signaling pathway
IF: 2.9 -
Severe hypoglycaemia-induced microglial inflammation damages microvascular endothelial cells, leading to retinal destruction
IF: 2.8 -
The impact of inflammatory and oxidative stress biomarkers on the sympathetic nervous system in severe coronary atherosclerosis
IF: 2.8Journal: Frontiers in Cardiovascular Medicine (2024)Reactivity:HumanSample Type:Peripheral venous blood sample

暂无文件,请在搜索框输入识别码查询相关文件

名称 | 状态 | 有效期至 | 操作 | |
---|---|---|---|---|
{{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注:请在有效期内下载相关文件,过期后页面内将不提供文件下载及预览!