Human IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Inflammatory Status of Patients with Dental Implants of Different Microgeometry using ELISA: A Prospective Clinical Study
IF: 0.5Journal： Journal of International Oral Health (2024)Reactivity：HumanSample Type：peri-implant crevicular fluid (PICF) -
Discovery and biological evaluation of a potent small molecule CRM1 inhibitor for its selective ablation of extranodal NK/T cell lymphoma
IF: 7.7 -
Gypenoside XVII inhibits ox-LDL-induced macrophage inflammatory responses and promotes cholesterol efflux through activating the miR-182–5P/HDAC9 signaling pathway
IF: 5.4 -
Identification of potential miR?155 target genes in epidermal immune microenvironment of atopic dermatitis patients and their inflammatory effects on HaCaT cells
IF: 2.7 -
The Inflammatory and Cytological Effect of Repeated Povidone-Iodine Application in Patients Receiving Intravitreal Injections
IF: 2.3Journal： Eye & Contact Lens-Science and Clinical Practice (2023)Reactivity：HumanSample Type：tear -
TRIM50 promotes NLRP3 inflammasome activation by directly inducing NLRP3 oligomerization
IF: 9.071 -
Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF
IF: 3.616 -
The Combination of Insulin Resistance and Serum Interleukin-1β Correlates with Post-Stroke Depression in Patients with Acute Ischemic Stroke
IF: 2.989Journal： Neuropsychiatric Disease and Treatment (2022)PMID：33727817Reactivity：humanSample Type：Serum -
Lipoxin A4 alleviates inflammation in Aspergillus fumigatus–stimulated human corneal epithelial cells by Nrf2/HO-1 signaling pathway
IF: 2.711 -
Inflammatory Markers Combined With Metalloproteinase-9, Neopterin, and S100B Concentrations May Indicate the Pathogenesis of Central Nervous System Diseases in Children
IF: 2.363Journal： JOURNAL OF CHILD NEUROLOGY (2022)Reactivity：HumanSample Type：Serum
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
