Human IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Early modulation of macrophage ROS-PPARγ-NF-κB signalling by sonodynamic therapy attenuates neointimal hyperplasia in rabbits
IF: 3.998Journal： Scientific Reports (2020)PMID：32669704Reactivity：HumanSample Type：Cell culture supernatant -
IL-38 alleviates the inflammatory response and the degeneration of nucleus pulposus cells via inhibition of the NF-κB signaling pathway in vitro
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：32502922Reactivity：HumanSample Type：Cell culture supernatant -
Mammalian Ste20-like kinase 4 inhibits the inflammatory response in Aspergillus fumigatus keratitis
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：33182037Reactivity：HumanSample Type：Cell lysate -
Construction of an exposure-pathway-phenotype in children with depression due to transfusion-dependent thalassemia: Results of (un)supervised machine learning.
IF: 3.892 -
Anti-angiogenic effect of Shikonin in rheumatoid arthritis by downregulating PI3K/AKT and MAPKs signaling pathways
IF: 3.690Journal： JOURNAL OF ETHNOPHARMACOLOGY (2020)PMID：32497675Reactivity：HumanSample Type：Serum,Cell culture supernatant -
Nec-1 Attenuates Neurotoxicity Induced by Titanium Dioxide Nanomaterials on Sh-Sy5y Cells Through RIP1
IF: 3.578 -
iTRAQ-based proteomics and in vitro experiments reveals essential roles of ACE and AP-N in the renin–angiotensin system-mediated congenital ureteropelvic junction obstruction
IF: 3.383Journal： EXPERIMENTAL CELL RESEARCH (2020)PMID：32416091Reactivity：HumanSample Type：Serum,Cell sample -
GPR120 Ameliorates Apoptosis and Inhibits the Production of Inflammatory Cytokines in Renal Tubular Epithelial Cells
IF: 3.212 -
MiR-1202 Exerts Neuroprotective Effects on OGD/R Induced Inflammation in HM Cell by Negatively Regulating Rab1a Involved in TLR4/NF-κB Signaling Pathway
IF: 3.038Journal： NEUROCHEMICAL RESEARCH (2020)PMID：32124161Reactivity：HumanSample Type：Cell culture medium -
MiR-942-5p alleviates septic acute kidney injury by targeting FOXO3
IF: 3.024Journal： European Review for Medical and Pharmacological Sciences (2020)PMID：32572890Reactivity：HumanSample Type：Cell culture supernatant
实验操作视频
