Human IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Lowered serum cesium levels in schizophrenia: association with immune-inflammatory biomarkers and cognitive impairments
IF: 1.904 -
HMGB-1, TLR4, IL-1R1, TNF-α, and IL-1β: novel epilepsy markers?
IF: 1.276 -
Molecular characterization, inducible expression and functional analysis of an IKKβ from the tropical sea cucumber Holothuria leucospilota
Journal： FISH & SHELLFISH IMMUNOLOGY (2020)PMID：32585358Reactivity：HumanSample Type：Cell culture supernatant -
Increased Liver Localization of Lipopolysaccharides in Human and Experimental NAFLD
IF: 14.971 -
GSTO1*CC Genotype (rs4925) Predicts Shorter Survival in Clear Cell Renal Cell Carcinoma Male Patients
IF: 6.162Journal： Cancers (2019)PMID：31861116Reactivity：HumanSample Type：Serum,Plasma,Whole Blood,Platelet -
Inhibition of CREB-mediated ZO-1 and activation of NF-κB-induced IL-6 by colonic epithelial MCT4 destroys intestinal barrier function
IF: 5.039 -
Targeting the IL-1β/IL-1Ra pathways for the aggregation of human islet amyloid polypeptide in an ex vivo organ culture system of the intervertebral disc
IF: 4.743 -
Arsenic Trioxide in Synergy with Vitamin D Rescues the Defective VDR-PPAR- Functional Module of Autophagy in Rheumatoid Arthritis
IF: 4.186 -
Tocilizumab attenuates acute lung and kidney injuries and improves survival in a rat model of sepsis via down-regulation of NF-κB/JNK: a possible role of P-glycoprotein
IF: 3.838 -
Sapylin (OK-432) alters inflammation and angiogenesis in vivo and vitro
IF: 3.743Journal： BIOMEDICINE & PHARMACOTHERAPY (2019)PMID：30844656Reactivity：HumanSample Type：Wound drainage fluid
实验操作视频
