Human IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
XCL1 Aggravates Diabetic Nephropathy-Mediated Renal Glomerular Endothelial Cell Apoptosis and Inflammatory Response via Regulating p53/Nuclear Factor-Kappa B Pathway
IF: 2.847 -
The effect of conventional versus electronic cigarette use on treatment outcomes of peri-implant disease
IF: 2.757 -
The Anti-Inflammatory Effect of Smilax china L. Extract on LPS-Stimulated THP-1 via Downregulation of MAPK and NF- κ B Signaling Pathway
IF: 2.630Journal： Evidence-based Complementary and Alternative Medicine (2021)PMID：34824594Reactivity：Human -
Increased Frequency of Circulating Classical Monocytes in Patients with Rosacea
IF: 2.489Journal： Clinical Cosmetic and Investigational Dermatology (2021)Reactivity：HumanSample Type：Plasma -
MiR-223-3p and miR-22-3p inhibit monosodium urate-induced gouty inflammation by targeting NLRP3
IF: 2.454Journal： International Journal of Rheumatic Diseases (2021)PMID：33650318Reactivity：HumanSample Type：Cell culture supernatant(THP-1) -
MicroRNA?16 regulates lipopolysaccharide?induced inflammatory factor expression by targeting TLR4 in normal human bronchial epithelial cells
IF: 2.447 -
Mangiferin inhibits hypoxia/reoxygenation?induced alveolar epithelial cell injury via the SIRT1/AMPK signaling pathway
IF: 2.447 -
Total Flavonoids of Engelhardia roxburghiana Wall. Leaves Alleviated Foam Cells Formation through AKT/mTOR-Mediated Autophagy in the Progression of Atherosclerosis
IF: 2.408 -
Exploring potential serum levels of Homocysteine, interleukin-1 beta, and apolipoprotein B 48 as new biomarkers for patients with ischemic stroke
IF: 2.352Journal： JOURNAL OF CLINICAL LABORATORY ANALYSIS (2021)PMID：34492129Reactivity：HumanSample Type：serum -
Analysis of serum cytokine and protective vitamin D levels in severe cases of COVID-19
IF: 2.327
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
