Human IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Association of PAR-2 Gene Polymorphisms with the Inflammatory Response and Susceptibility to Knee Osteoarthritis in the Chinese Han Population
IF: 1.121Journal： Genetic Testing and Molecular Biomarkers (2019)PMID：30688539Reactivity：HumanSample Type：Synovia -
The enhancer RNA lnc-SLC4A1-1 epigenetically regulates unexplained recurrent pregnancy loss (URPL) by activating CXCL8 and NF-kB pathway
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Prostaglandin E2/EP2 receptor signalling pathway promotes diabetic retinopathy in a rat model of diabetes
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miR-93-5p-Containing Exosomes Treatment Attenuates Acute Myocardial Infarction-Induced Myocardial Damage
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Increased Expression of TLR10 in B Cell Subsets Correlates with Disease Activity in Rheumatoid Arthritis
IF: 3.549 -
RETRACTED: LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203
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Molecular characterization and functional analysis of MyD88 from the tropical sea cucumber, Holothuria leucospilota
IF: 3.185 -
Novel anti-inflammatory target of geniposide: Inhibiting Itgβ1/Ras-Erk1/2 signal pathway via the miRNA-124a in rheumatoid arthritis synovial fibroblasts
IF: 3.118 -
S1P promotes inflammation-induced tube formation by HLECs via the S1PR1/NF-κB pathway
IF: 3.118Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2018)PMID：30476824Reactivity：HumanSample Type：Cell culture supernatant -
Exosomes derived from human umbilical cord mesenchymal stem cells alleviate acute liver failure by reducing the activity of the NLRP3 inflammasome in macrophages
IF: 2.559Journal： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2018)PMID：30528233Reactivity：HumanSample Type：Serum
实验操作视频
