Human IL-1β(Interleukin 1 Beta) ELISA Kit
中文名称：人白介素1β(IL-1β)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-1β抗体包被于酶标板上，实验时样品（或标准品）中的人IL-1β会与包被抗体结合。后依次加入生物素化的抗人IL-1β抗体和辣根过氧化物酶标记的亲和素，抗人IL-1β抗体与结合在包被抗体上的人IL-1β结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-1β浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-1β的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-1β，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
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Fortunellin attenuates sepsis-induced acute kidney injury by inhibiting inflammation and ferroptosis via the TLR4/NF-κB pathway
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White Tea Consumption Alleviates Anthropometric and Metabolic Parameters in Obese Patients
IF: 2.400 -
Silencing LncRNA SNHG14 alleviates renal tubular injury via the miR-483-5p/HDAC4 axis in diabetic kidney disease
IF: 2.400Journal： Hormones-International Journal of Endocrinology and Metabolism (2024)Reactivity：Human -
Depression of LncRNA DANCR alleviates tubular injury in diabetic nephropathy by regulating KLF5 through sponge miR-214-5p
IF: 2.200 -
Evaluation of inflammasomes as biomarker following non-surgical periodontal treatment
IF: 2.200Journal： ARCHIVES OF ORAL BIOLOGY (2024)Reactivity：HumanSample Type：saliva ,serum -
Exploring the Association between Depression, Suicidality, and Serum Brain-Derived Neurotrophic Factor, FAM19A5, Total Antioxidant Status, Total Oxidant Status, Oxidative Stress Index, and Cytokine Levels: A Case-Control Study of Drug-Naive Adolescents with First-Episode Major Depressive Disorder
IF: 1.900 -
Based on PI3K/Akt Signaling Pathway, the Effects of Gubi Decoction on Chondrocyte Proliferation, Apoptosis, and Expression of Inflammatory Factors in a Rat Model of Osteoarthritis.
IF: 1.900Journal： ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE (2024)DOI：PMID:39038323Reactivity：RatSample Type：serum -
Epigallocatechin-3-gallate alleviates ethanol-induced endothelia cells injury partly through alteration of NF-κB translocation and activation of the Nrf2 signaling pathway
IF: 1.700 -
IL-27 attenuated macrophage injury and inflammation induced by Mycobacterium tuberculosis by activating autophagy
IF: 1.500Journal： IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2024)Reactivity：Human
实验操作视频
