Human IL-22(Interleukin 22) ELISA Kit
中文名称：人白介素22(IL-22)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-22抗体包被于酶标板上，实验时样品（或标准品）中的人IL-22会与包被抗体结合。后依次加入生物素化的抗人IL-22抗体和辣根过氧化物酶标记的亲和素，抗人IL-22抗体与结合在包被抗体上的人IL-22结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-22浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-22的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-22，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Immune pathogenesis of idiopathic granulomatous mastitis: from etiology toward therapeutic approaches
IF: 7.300Journal： Frontiers in Immunology (2024)PMID：38529282Reactivity：HumanSample Type：Idiopathic granulomatous mastitis (IGM) -
Profile of immune response during nasal challenge with dermatophagoides pteronyssinus in subjects with allergic airway diseases
IF: 4.400Journal： Journal of Inflammation-London (2024)Reactivity：HumanSample Type：serum,nasal lavage tissue -
Keratinocyte exosomal LOC285194 ameliorates psoriasis by inhibiting the differentiation of CD4+T cells to Th17 cells through regulating miR-211-5p/SIRT1 axis
IF: 3.700 -
Interleukin-22 promotes endometrial carcinoma cell proliferation and cycle progression via ERK1/2 and p38 activation
IF: 3.500Journal： MOLECULAR AND CELLULAR BIOCHEMISTRY (2024)Reactivity：HumanSample Type：serum -
Cosentyx alleviates psoriasis-induced podocyte injury by inhibiting the tlr/nf-κb signaling pathway
IF: 2.200 -
Downregulation of circETS1 disrupts Th17/Treg homeostasis by inhibiting FOXP3 transcription: A new potential biomarker in systemic lupus erythematosus
IF: 2.600 -
The effect of secukinumab treatment for psoriasis on serum cytokines and correlation with disease severity
IF: 2.200 -
The lncRNA H19/miR-766-3p/S1PR3 Axis Contributes to the Hyperproliferation of Keratinocytes and Skin Inflammation in Psoriasis via the AKT/mTOR Pathway
IF: 4.711 -
Role of IL-22 in persistent allergic airway diseases caused by house dust mite: a pilot study
IF: 3.317Journal： BMC Pulmonary Medicine (2021)PMID：33478443Reactivity：HumanSample Type：Serum,Nasal Lavage Fluid -
Systemic and local cytokine profile and risk factors for persistent allergic airway inflammation in patients sensitised to house dust mite allergens
IF: 3.317Journal： BMC Pulmonary Medicine (2021)PMID：34930201Reactivity：HumanSample Type：serum, nasal lavage
实验操作视频
