Human IL-33(Interleukin 33) ELISA Kit
中文名称：人白介素33(IL-33)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-33抗体包被于酶标板上，实验时样品（或标准品）中的人IL-33会与包被抗体结合。后依次加入生物素化的抗人IL-33抗体和辣根过氧化物酶标记的亲和素，抗人IL-33抗体与结合在包被抗体上的人IL-33结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-33浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-33的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-33，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
The Alarmin Triad—IL-25, IL-33, and TSLP—Serum Levels and Their Clinical Implications in Chronic Spontaneous Urticaria
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)PMID：38396704Reactivity：HumanSample Type：serumRelation of T Cell Profile with Vitamin D Receptor and Vitamin D-Binding Protein Gene Polymorphisms in Atopy
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：HumanSample Type：SerumProfile of immune response during nasal challenge with dermatophagoides pteronyssinus in subjects with allergic airway diseases
IF: 4.400Journal： Journal of Inflammation-London (2024)Reactivity：HumanSample Type：serum,nasal lavage tissueSulforaphane inhibits the migration and invasion of BPDE-induced lung adenocarcinoma cells by regulating NLRP12
IF: 3.800Biomarkers of type 2 and non-type 2 inflammation in asthma exacerbations
IF: 1.500Journal： Central European Journal of Immunology (2024)Reactivity：HumanSample Type：serumSUMOylated IL-33 in the nucleus stabilizes the transcription factor IRF1 in hepatocellular carcinoma cells to promote immune escape
IF: 7.300Salivary IL-33 and sST2 levels in relation to TLR2 rs111200466 polymorphism and periodontitis
IF: 3.800Prognostic value of interleukin-33, sST2, myeloperoxidase, and matrix metalloproteinase-9 in acute aortic dissection
IF: 3.600Journal： Frontiers in Cardiovascular Medicine (2023)PMID：36684579Reactivity：HumanSample Type：plasmaA role for serum cytokines and cell adhesion molecules in the non?invasive diagnosis of colorectal cancer
IF: 3.111The function of IL-33/ST2 signaling axis in treg cells activating fibrosis in IgG4-related disease
IF: 2.211
