Human IL-4(Interleukin 4) ELISA Kit
中文名称：人白介素4(IL-4)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 31.25-2000 pg/mL
- 灵敏度： 18.75 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-4抗体包被于酶标板上，实验时样品（或标准品）中的人IL-4会与包被抗体结合。后依次加入生物素化的抗人IL-4抗体和辣根过氧化物酶标记的亲和素，抗人IL-4抗体与结合在包被抗体上的人IL-4结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-4浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-4的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 31.25-2000 pg/mL |
| 灵敏度 | 18.75 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-4，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Decreased CXCR2 expression on circulating monocytes of colorectal cancer impairs recruitment and induces Re-education of tumor-associated macrophages
IF: 9.756 -
Genipin Attenuates Diabetic Cognitive Impairment by Reducing Lipid Accumulation and Promoting Mitochondrial Fusion via FABP4/Mfn1 Signaling in Microglia
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Different oral and gut microbial profiles in those with Alzheimer's disease consuming anti-inflammatory diets
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Berberine Inhibits the Adhesion of Candida albicans to Vaginal Epithelial Cells
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IF: 5.732Journal： CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2022)PMID：35348596Reactivity：HumanSample Type：blood -
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IF: 5.657 -
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?
IF: 3.657Journal： JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022)PMID：36179036Reactivity：HumanSample Type：fecal -
Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF
IF: 3.616 -
Regulatory Effects of Fyn on Trophoblast Cell Behaviors and Function
IF: 3.246
实验操作视频
