Human IL-5(Interleukin 5) ELISA Kit
中文名称：人白介素5(IL-5)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-5抗体包被于酶标板上，实验时样品（或标准品）中的人IL-5会与包被抗体结合。后依次加入生物素化的抗人IL-5抗体和辣根过氧化物酶标记的亲和素，抗人IL-5抗体与结合在包被抗体上的人IL-5结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-5浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-5的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-5，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
M2 macrophages participate in ILC2 activation induced by Helicobacter pylori infection
IF: 12.200 -
The effect of combining an inhaled corticosteroid and a long-acting muscarinic antagonist on human airway epithelial cells in vitro
IF: 5.800 -
Relation of T Cell Profile with Vitamin D Receptor and Vitamin D-Binding Protein Gene Polymorphisms in Atopy
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：HumanSample Type：Serum -
An ayurvedic approach to immunomodulation employing a green delivery of phytonutrients: A randomized, double-blind, placebo-controlled study on mild allergic rhinitis
IF: 3.800Journal： Journal of Functional Foods (2024)Reactivity：HumanSample Type：Serum -
The association of 25(OH)D, interleukin-4, interleukin-5, and interleukin-13 levels with the burden of soil-transmitted helminth infection in stunted children aged 24–59 months
IF: 3.500 -
Surveillance of Soil-Transmitted Helminth Infection in Preschool Child Population: Do Changes in Behavior and Immunological Responses Affect Prevalence?
IF: 2.900Journal： Tropical Medicine and Infectious Disease (2024)PMID：38393122Reactivity：HumanSample Type：blood -
Inflammatory markers in exhaled breath condensate in nonasthmatic children with food allergy
IF: 3.800Journal： Journal of Breath Research (2023)PMID：37827149Reactivity：HumanSample Type：Exhaled breath condensate (EBC) samples -
Prolonged SIRS after cardiac surgery
IF: 2.800Journal： JOURNAL OF CARDIOTHORACIC AND VASCULAR ANESTHESIA (2023)PMID：38220516Reactivity：HumanSample Type：serum -
Neutrophil membrane-camouflaged nanoparticles alleviate inflammation and promote angiogenesis in ischemic myocardial injury
IF: 16.874 -
Clinical features and nasal inflammation in asthma and allergic rhinitis
IF: 5.732Journal： CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2022)PMID：35348596Reactivity：HumanSample Type：blood
实验操作视频
