Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Serum mir-142-3p release in children with viral encephalitis and its relationship with nerve injury and inflammatory response
IF: 2.300Journal： JOURNAL OF NEUROVIROLOGY (2024)Reactivity：HumanSample Type：serum -
Cosentyx alleviates psoriasis-induced podocyte injury by inhibiting the tlr/nf-κb signaling pathway
IF: 2.200 -
Prognostic potency of plasma LRG1 measurement at multiple time points in acute ischemic stroke patients
IF: 2.200 -
Depression of LncRNA DANCR alleviates tubular injury in diabetic nephropathy by regulating KLF5 through sponge miR-214-5p
IF: 2.200 -
Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis
IF: 2.200Journal： ARCHIVES OF ORAL BIOLOGY (2024)Reactivity：HumanSample Type：gingival crevicular fluid (GCF) sample -
Antiaging properties of chlorogenic acid through protein and gene biomarkers in human skin fibroblast cells as photoaging model
IF: 2.100 -
Investigation of the Potential Targets and Mechanism Actions of Berberine in the Treatment of Pulpitis Based on Bioinformatics Analysis
IF: 2.000Journal： JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS (2024)Reactivity：Human -
GRP78 mediates mitochondrial fusion and fission in cigarette smoke-induced inflammatory responses in airway epithelial cells
IF: 2.000 -
Exploring the Association between Depression, Suicidality, and Serum Brain-Derived Neurotrophic Factor, FAM19A5, Total Antioxidant Status, Total Oxidant Status, Oxidative Stress Index, and Cytokine Levels: A Case-Control Study of Drug-Naive Adolescents with First-Episode Major Depressive Disorder
IF: 1.900 -
Effect of Millet Polyphenol-Rich Extracts on Human Fecal Bacteria and Dextran Sulfate Sodium-Induced Human Intestinal Epithelial Cell Derangements
IF: 1.800
实验操作视频
