Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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ClinOleic Impairs ROS Production and Phagocytosis in M1 Macrophages Without Affecting M1 Differentiation
IF: 1.800 -
Liquiritin Alleviates Inflammation in Lipopolysaccharide-Induced Human Corneal Epithelial Cells
IF: 1.700 -
Epigallocatechin-3-gallate alleviates ethanol-induced endothelia cells injury partly through alteration of NF-κB translocation and activation of the Nrf2 signaling pathway
IF: 1.700 -
Anesthesia Medication’s Impacts on Inflammatory and Neuroendocrine Immune Response in Patients Undergoing Digestive Endoscopy
IF: 1.700Journal： Clinics and Practice (2024)Reactivity：HumanSample Type：serum -
ACSL4 mediates inflammatory bowel disease and contributes to LPS-induced intestinal epithelial cell dysfunction by activating ferroptosis and inflammation
IF: 1.700 -
IL-27 attenuated macrophage injury and inflammation induced by Mycobacterium tuberculosis by activating autophagy
IF: 1.500Journal： IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL (2024)Reactivity：Human -
CircYTHDF1/miR-19b-3p/YTHDF1 axis contributes to pregnancy-induced hypertension development by enhancing vascular endothelial cell injury
IF: 1.500Journal： HYPERTENSION IN PREGNANCY (2024)Reactivity：HumanSample Type：peripheral blood -
Predictive value of?miR-582-5p for onset of?sepsis-induced acute kidney injury and its functional role during disease development
IF: 1.500Journal： Central European Journal of Immunology (2024)Reactivity：Human -
Muscone Abates 1,2-Dimethylhydrazine-Induced Colon Cancer in Rats and HCT-116 Cells Proliferation via Modulation of TNF-α, COX-2, NF-kB, and Phase I Xenobiotic Enzymes
IF: 1.400Journal： Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy (2024)Reactivity：Human -
Effects of low fat diet on inflammatory parameters in individuals with obesity/overweight and non-alcoholic fatty liver disease: A cross-sectional study
IF: 1.300
实验操作视频
