Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Fluorometholone inhibits high glucose-induced cellular senescence in human retinal endothelial cells
IF: 3.247 -
Association between pro-inflammatory cytokines in cerebrospinal fluid and headache in patients with aneurysmal subarachnoid hemorrhage
IF: 3.221Journal： JOURNAL OF NEUROIMMUNOLOGY (2022)PMID：35279426Reactivity：HumanSample Type：cerebrospinal fluid (CSF) -
Role of CD36 rs1761667 AA genotype in the expression of inflammatory cytokines and Parkinson’s disease progression: A case-control study in a northern Han Chinese population
IF: 3.197 -
A role for serum cytokines and cell adhesion molecules in the non?invasive diagnosis of colorectal cancer
IF: 3.111 -
Serum Sirtuin-1, HMGB1-TLR4, NF-KB and IL-6 Levels in Alzheimer’s: The Relation Between Neuroinflammatory Pathway and Severity of Dementia
IF: 3.040Journal： Current Alzheimer Research (2022)Reactivity：HumanSample Type：serum -
Interleukin-6 in Cerebrospinal Fluid Small Extracellular Vesicles as a Potential Biomarker for Prognosis of Aneurysmal Subarachnoid Haemorrhage
IF: 2.989Journal： Neuropsychiatric Disease and Treatment (2022)PMID：34012263Reactivity：HumanSample Type：Cerebrospinal Fluid -
Differential expression of RNA-binding proteins in bronchial epithelium of stable COPD patients
IF: 2.893Journal： International Journal of Chronic Obstructive Pulmonary Disease (2022)PMID：30349226Reactivity：Human -
Periodontal Ligament Stem Cells Reverse the High Glucose Level-Induced Inflammatory State of Macrophages
IF: 2.831 -
Role of the long non-coding RNA, SPRR2C, based on an in vitro psoriatic keratinocyte cell model
IF: 2.805 -
Lipoxin A4 alleviates inflammation in Aspergillus fumigatus–stimulated human corneal epithelial cells by Nrf2/HO-1 signaling pathway
IF: 2.711
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