Human IL-6(Interleukin 6) ELISA Kit
中文名称:人白介素6(IL-6)酶联免疫吸附测定试剂盒

Price:
- 反应性: Human
- 检测范围: 1.56-100 pg/mL
- 灵敏度: 0.94 pg/mL
用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上,实验时样品或标准品中的人IL-6会与包被抗体结合,游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450nm波长处测OD值,IL-6浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中IL-6的浓度。 |
反应类型 | Sandwich-ELISA |
规格 |
96 T
/ 48 T
/ 96 T × 5
|
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 pg/mL |
灵敏度 | 0.94 pg/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人IL-6,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
-
Circ_0043947 contributes to interleukin 1β-induced injury in chondrocytes by sponging miR-671-5p to up-regulate RTN3 expression in osteoarthritis pathology
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Circ_0101874 overexpression strengthens PDE4D expression by targeting miR-335-5p to promote neuronal injury in ischemic stroke
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Effect of Thymosin on Inflammatory Factor Levels, Immune Function, and Quality of Life in Lung Cancer Patients Undergoing Radical Thoracoscopic Surgery
IF: 2.650Journal: Evidence-based Complementary and Alternative Medicine (2022)PMID:35832513Reactivity:HumanSample Type:Serum -
Febuxostat Alleviates Allergic Rhinitis by Inhibiting Inflammation and Monocyte Adhesion in Human Nasal Epithelial Cells via Regulating KLF6.
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Immune Cell and Biochemical Biomarkers in Advanced Laryngeal Cancer
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CFNAs of RBCs affect the release of inflammatory factors through the expression of CaMKIV in macrophages
IF: 2.596 -
Upregulated NORAD is implicated in apoptosis, inflammation, and oxidative stress in ulcerative colitis through the nuclear factor-κappaB signaling
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The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis.
IF: 2.585Journal: Canadian Journal of Infectious Diseases & Medical Microbiology (2022)PMID:35873363Reactivity:Human -
Association of the IRAK4 rs4251545 genetic polymorphism with severity of enterovirus-71 infection in Chinese children
IF: 2.493Journal: Immunity Inflammation and Disease (2022)DOI:10.1002/iid3.614PMID:35478439Reactivity:HumanSample Type:plasma

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