Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Preparation and characterization of cysteine-rich collagen peptide and its antagonistic effect on microplastic induced damage to HK-2?cells
IF: 4.800 -
Trim31 deficiency exacerbates airway inflammation in asthma by enhancing the activation of the NLRP3 inflammasome
IF: 4.800 -
Regulation mechanism of GPS2 on PGC-1α/Drp1-mediated mitochondrial dynamics in inflammation of acute lung injury
IF: 4.800 -
Effect of Fermented Soybean (FSB) Supplementation on Gastroesophageal Reflux Disease (GERD)
IF: 4.800 -
A synergistic effect of triptolide and curcumin on rheumatoid arthritis by improving cell proliferation and inducing cell apoptosis via inhibition of the IL-17/NF-κB signaling pathway
IF: 4.800 -
Injectable Tannin-Containing Hydroxypropyl Chitin Hydrogel as Novel Bioactive Pulp Capping Material Accelerates Repair of Inflamed Dental Pulp
IF: 4.800 -
Tumor-associated macrophages induce epithelial-mesenchymal transition and promote lung metastasis in breast cancer by activating the IL-6/STAT3/TGM2 axis
IF: 4.800 -
Influence of CReatine Supplementation on mUScle Mass and Strength After Stroke (ICaRUS Stroke Trial): A Randomized Controlled Trial
IF: 4.800 -
Role of Increasing Body Mass Index in Gut Barrier Dysfunction, Systemic Inflammation, and Metabolic Dysregulation in Obesity
IF: 4.800 -
Regulatory Effect of Fucoidan Hydrolysates on Lipopolysaccharide-Induced Inflammation and Intestinal Barrier Dysfunction in Caco-2 and RAW264.7 Cells Co-Cultures
IF: 4.700
实验操作视频
