Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Short?term efficacy and safety of prednisone in herpes zoster and the effects on IL?6 and IL?10
IF: 1.448Journal： Experimental and Therapeutic Medicine (2019)PMID：31572533Reactivity：HumanSample Type：serum -
Systematically transplanted human gingiva?derived mesenchymal stem cells regulate lipid metabolism and inflammation in hyperlipidemic mice with periodontitis
IF: 1.448Journal： Experimental and Therapeutic Medicine (2019)PMID：31885706Reactivity：HumanSample Type：Serum -
Changes in expression of serum chemokine CXCL13 and IL-6 after hip replacement, and the relationship with lower limb vein thrombus
IF: 1.448Journal： Experimental and Therapeutic Medicine (2019)PMID：32104273Reactivity：HumanSample Type：Serum -
Association of PAR-2 Gene Polymorphisms with the Inflammatory Response and Susceptibility to Knee Osteoarthritis in the Chinese Han Population
IF: 1.121Journal： Genetic Testing and Molecular Biomarkers (2019)PMID：30688539Reactivity：HumanSample Type：Synovia -
Interleukin 8 as predictive factor for response to chemotherapy in colorectal cancer patients
IF: 0.960 -
TLR7 mediates increased vulnerability to ischemic acute kidney injury in diabetes
IF: 0.801Journal： Revista da Associacao Medica Brasileira (2019)PMID：31531603Reactivity：HumanSample Type：Cell culture supernatant -
The Effects of a Single Versus Three Consecutive Sessions of Football Training on Postprandial Lipemia: a Randomized, Controlled Trial in Healthy, Recreationally Active Males
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miR-93-5p-Containing Exosomes Treatment Attenuates Acute Myocardial Infarction-Induced Myocardial Damage
IF: 5.660 -
Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis
IF: 5.454 -
Mesenchymal Stem Cells (MSCs) Coculture Protects [Ca2+]i Orchestrated Oxidant Mediated Damage in Differentiated Neurons In Vitro
IF: 4.829
实验操作视频
