Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
MicroRNA-101-3p Downregulates TLR2 Expression, Leading to Reduction in Cytokine Production by Treponema pallidum–Stimulated Macrophages
IF: 7.143Journal： JOURNAL OF INVESTIGATIVE DERMATOLOGY (2020)PMID：31930972Reactivity：HumanSample Type：plasma -
PM2.5 organic extract mediates inflammation through the ERβ pathway to contribute to lung carcinogenesis in?vitro and vivo
IF: 5.778 -
Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils
IF: 5.546Journal： JOURNAL OF CELLULAR PHYSIOLOGY (2020)PMID：32385929Reactivity：HumanSample Type：Cell culture supernatant -
Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function
IF: 5.223Journal： CLINICAL SCIENCE (2020)PMID：32219341Reactivity：HumanSample Type：Cell culture supernatant -
EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma
IF: 5.186Journal： Frontiers in Cell and Developmental Biology (2020)PMID：33363140Reactivity：HumanSample Type：Tissue homogenate -
Fontan protein-losing enteropathy is associated with advanced liver disease and a proinflammatory intestinal and systemic state
IF: 5.175 -
Oxidative Stress and Pro-Inflammatory Status in Patients with Non-Alcoholic Fatty Liver Disease
IF: 5.014 -
CALML6 Controls TAK1 Ubiquitination and Confers Protection against Acute Inflammation
IF: 4.886Journal： JOURNAL OF IMMUNOLOGY (2020)PMID：32303555Reactivity：HumanSample Type：Cell culture supernatant -
Short-Term Physiological Effects of a Very Low-Calorie Ketogenic Diet: Effects on Adiponectin Levels and Inflammatory States
IF: 4.556Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)PMID：32370212Reactivity：HumanSample Type：serum -
LncRNA SNHG5 regulates cell apoptosis and inflammation by miR-132/PTEN axis in COPD
IF: 4.545Journal： BIOMEDICINE & PHARMACOTHERAPY (2020)PMID：32145584Reactivity：HumanSample Type：Cell culture supernatant
暂无文件，请在搜索框输入识别码查询相关文件
| 名称 | 状态 | 有效期至 | 操作 | |
|---|---|---|---|---|
| {{item.文件名}} | {{item.status_str}} | {{item.timestamp_str}} | 点击查看 点击预览 |
*注：请在有效期内下载相关文件，过期后页面内将不提供文件下载及预览！
实验操作视频
