Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Chrysin protects human osteoarthritis chondrocytes by inhibiting inflammatory mediator expression via HMGB1 suppression
IF: 1.922Journal： Molecular Medicine Reports (2018)PMID：30535473Reactivity：HumanSample Type：Cell culture medium -
Propofol Promotes Ankle Fracture Healing in Children by Inhibiting Inflammatory Response
IF: 1.894 -
Sinomenine Regulates Inflammatory Response and Oxidative Stress via Nuclear Factor kappa B (NF-κB) and NF-E2-Related Factor 2 (Nrf2) Signaling Pathways in Ankle Fractures in Children
IF: 1.894 -
N?methyl pyrrolidone promotes ankle fracture healing by inhibiting inflammation via suppression of the mitogen?activated protein kinase signaling pathway
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：29545891Reactivity：HumanSample Type：peripheral blood -
Anti?inflammatory effect of Resolvin D1 on LPS?treated MG?63 cells Corrigendum in /10.3892/etm.2019.8021
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30402165Reactivity：HumanSample Type：Serum -
MicroRNA-124 alleviates hyperoxia-induced inflammatory response in pulmonary epithelial cell by inhibiting TLR4/NF-κB/CCL2
IF: 1.396Journal： International Journal of Clinical and Experimental Pathology (2018)DOI：PMID:31938089PMID：31938089Reactivity：Human -
Overcoming the Heat Endurance of Tumor Cells by Interfering with the Anaerobic Glycolysis Metabolism for Improved Photothermal Therapy
IF: 13.942 -
Multifunctional Dextran Sulfate-Coated Reconstituted High Density Lipoproteins Target Macrophages and Promote Beneficial Antiatherosclerotic Mechanisms
IF: 4.818Journal： BIOCONJUGATE CHEMISTRY (2017)PMID：28004910Reactivity：HumanSample Type：Cell culture supernatant -
The classic signalling and trans-signalling of interleukin-6 are both injurious in podocyte under high glucose exposure
IF: 4.499Journal： JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2017)PMID：28881473Reactivity：HumanSample Type：Serum -
Antibiotics induce polarization of pleural macrophages to M2-like phenotype in patients with tuberculous pleuritis
IF: 4.259Journal： Scientific Reports (2017)PMID：29101376Reactivity：HumanSample Type：tuberculous pleural effusion
实验操作视频
