Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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MicroRNA-218 acts by repressing TNFR1-mediated activation of NF-κB, which is involved in MUC5AC hyper-production and inflammation in smoking-induced bronchiolitis of COPD
IF: 3.858Journal： TOXICOLOGY LETTERS (2017)PMID：28864214Reactivity：HumanSample Type：Serum,Cell culture supernatant -
High anti-human cytomegalovirus antibody levels are associated with the progression of essential hypertension and target organ damage in Han Chinese population
IF: 2.806 -
Depot-specific inflammation with decreased expression of ATM2 in white adipose tissues induced by high-margarine/lard intake
IF: 2.806 -
Oleanolic acid protects against pathogenesis of atherosclerosis, possibly via FXR-mediated angiotensin (Ang)-(1–7) upregulation
IF: 2.759Journal： BIOMEDICINE & PHARMACOTHERAPY (2017)PMID：29793333Reactivity：HumanSample Type：Cell culture supernatant,Serum -
C reactive protein and enzymatically modified LDL cooperatively promote dendritic cell-mediated T cell activation
IF: 2.359Journal： CARDIOVASCULAR PATHOLOGY (2017)PMID：28433861Reactivity：HumanSample Type：Cell culture supernatant -
Particulate matter 2.5 regulates lipid synthesis and inflammatory cytokine production in human SZ95 sebocytes
IF: 2.341Journal： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2017)PMID：28849137Reactivity：HumanSample Type：Cell culture supernatant -
Tetrahydroxy stilbene glucoside ameliorates H2O2-induced human brain microvascular endothelial cell dysfunction in?vitro by inhibiting oxidative stress and inflammatory responses
IF: 1.692Journal： Molecular Medicine Reports (2017)PMID：28849141Reactivity：HumanSample Type：Cell culture supernatant -
Inhibition of miR?27a suppresses the inflammatory response via the p38/MAPK pathway in intervertebral disc cells
IF: 1.261Journal： Experimental and Therapeutic Medicine (2017)PMID：29067127Reactivity：HumanSample Type：Cell culture supernatant -
Blockade of 146b?5p promotes inflammation in atherosclerosis?associated foam cell formation by targeting TRAF6
IF: 1.261 -
Role of miR?589?3p in human lumbar disc degeneration and its potential mechanism
IF: 1.261Journal： Experimental and Therapeutic Medicine (2017)PMID：29434746Reactivity：HumanSample Type：Cell culture supernatant
实验操作视频
