Human IL-6(Interleukin 6) ELISA Kit
中文名称：人白介素6(IL-6)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 1.56-100 pg/mL
- 灵敏度： 0.94 pg/mL
| 用途 | 试剂盒用于体外定量检测人血清、血浆或其他生物体液中IL-6浓度 |
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-6抗体包被于酶标板上，实验时样品或标准品中的人IL-6会与包被抗体结合，游离的成分被洗去。依次加入生物素化的抗人IL-6抗体和辣根过氧化物酶标记的亲和素。抗人IL-6抗体与结合在包被抗体上的人IL-6结合、生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450nm波长处测OD值，IL-6浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-6的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 1.56-100 pg/mL |
| 灵敏度 | 0.94 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-6，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Applied value of monitoring serum hepcidin in differential diagnosis of infection versus tumor fevers
IF: 0.964Journal： Journal of Huazhong University of Science and Technology-Medical Sciences (2017)PMID：28397053Reactivity：HumanSample Type：Plasma -
STAT3 regulates cytokine expression in peripheral blood mononuclear cells from asthma patients
IF: 0.920 -
TLR3-/4-Priming Differentially Promotes Ca2+ Signaling and Cytokine Expression and Ca2+-Dependently Augments Cytokine Release in hMSCs
IF: 5.228Journal： Scientific Reports (2016)PMID：26980664Reactivity：HumanSample Type：Cell culture supernatant -
Clonorchis sinensis Co-infection Could Affect the Disease State and Treatment Response of HBV Patients
IF: 3.948Journal： PLoS Neglected Tropical Diseases (2016)PMID：27348302Reactivity：HumanSample Type：Serum,Cell culture supernatant -
Inhibitory effect of miR-125b on hepatitis C virus core protein-induced TLR2/MyD88 signaling in THP-1 cells
IF: 2.787Journal： WORLD JOURNAL OF GASTROENTEROLOGY (2016)PMID：27158204Reactivity：HumanSample Type：Cell culture supernatant -
Antidiabetic (type 2) effects of Lactobacillus G15 and Q14 in rats through regulation of intestinal permeability and microbiota
IF: 2.686 -
PLD4 promotes M1 macrophages to perform antitumor effects in colon cancer cells
IF: 2.486 -
In vitro antiproliferative, pro-apoptotic, antimetastatic and anti-inflammatory potential of 2,4-diacteylphloroglucinol (DAPG) by Pseudomonas aeruginosa strain FP10
IF: 3.685 -
TSG attenuates LPC-induced endothelial cells inflammatory damage through notch signaling inhibition
IF: 3.143Journal： IUBMB LIFE (2015)DOI：10.1002/iub.1458PMID：26662286Reactivity：HumanSample Type：Cell culture supernatant -
Mitochondrion-Targeted Peptide SS-31 Inhibited Oxidized Low-Density Lipoproteins-Induced Foam Cell Formation through both ROS Scavenging and Inhibition of Cholesterol Influx in RAW264.7 Cells
IF: 2.416
实验操作视频
