Human IL-8(Interleukin 8) ELISA Kit
中文名称：人白介素8(IL-8)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上，实验时样品（或标准品）中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素，抗人IL-8抗体与结合在包被抗体上的人IL-8结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-8浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-8的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-8，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Liquiritin Alleviates Inflammation in Lipopolysaccharide-Induced Human Corneal Epithelial Cells
IF: 1.700 -
Anesthesia Medication’s Impacts on Inflammatory and Neuroendocrine Immune Response in Patients Undergoing Digestive Endoscopy
IF: 1.700Journal： Clinics and Practice (2024)Reactivity：HumanSample Type：serum -
Impact of remimazolam on postoperative inflammatory markers and complications in thoracoscopic pulmonary lobectomy patients: A retrospective analysis
IF: 1.300Journal： MEDICINE (2024)Reactivity：HumanSample Type：Peripheral venous blood -
YKL-40 Knockdown Decreases Oxidative Stress Damage in Ovarian Granulosa Cells
IF: 1.100Journal： Genetic Testing and Molecular Biomarkers (2024)Reactivity：Human -
Discovery and biological evaluation of a potent small molecule CRM1 inhibitor for its selective ablation of extranodal NK/T cell lymphoma
IF: 7.700 -
Identification of potential miR?155 target genes in epidermal immune microenvironment of atopic dermatitis patients and their inflammatory effects on HaCaT cells
IF: 2.700 -
Exploring the “gene–protein–metabolite” network of coronary heart disease with phlegm and blood stasis syndrome by integrated multi-omics strategy
IF: 5.988Journal： Frontiers in Pharmacology (2022)Reactivity：HumanSample Type：Serum -
Advanced platelet-rich fibrin promotes the paracrine function and proliferation of adipose-derived stem cells and contributes to micro-autologous fat transplantation by modulating HIF-1α and VEGF
IF: 3.616 -
Resveratrol attenuates cigarette smoke extract induced cellular senescence in human airway epithelial cells by regulating the miR-34a/SIRT1/NF-κB pathway
IF: 1.817 -
A SCID mouse-human lung xenograft model of SARS-CoV-2 infection
IF: 11.556
实验操作视频
