Human IL-8(Interleukin 8) ELISA Kit
中文名称：人白介素8(IL-8)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上，实验时样品（或标准品）中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素，抗人IL-8抗体与结合在包被抗体上的人IL-8结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-8浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-8的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-8，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Mediators of extracellular matrix degradation and inflammation: A new team of possible biomarkers for oral squamous cell carcinoma stage
IF: 2.447Journal： Experimental and Therapeutic Medicine (2021)PMID：34194555Reactivity：HumanSample Type：Oral cancer tissue -
miR-1287-5p upregulation inhibits the EMT and pro-inflammatory cytokines in LPS-induced human nasal epithelial cells (HNECs)
IF: 1.708 -
Comparison of Inflammation Biomarkers among Chronic Obstructive Pulmonary Disease Groups: A Cross Sectional Study
IF: 0.968Journal： Nigerian Journal of Clinical Practice (2021)Reactivity：HumanSample Type：serum -
Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output
IF: 38.637 -
Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils
IF: 5.546Journal： JOURNAL OF CELLULAR PHYSIOLOGY (2020)PMID：32385929Reactivity：HumanSample Type：Cell culture supernatant -
Effects of circulating extracellular microvesicles from spinal cord-injured adults on endothelial cell function
IF: 5.223Journal： CLINICAL SCIENCE (2020)PMID：32219341Reactivity：HumanSample Type：Cell culture supernatant -
EZH2-Inhibited MicroRNA-454-3p Promotes M2 Macrophage Polarization in Glioma
IF: 5.186Journal： Frontiers in Cell and Developmental Biology (2020)PMID：33363140Reactivity：HumanSample Type：Tissue homogenate -
Effect of Lactobacillus acidophilus Fermented Broths Enriched with Eruca sativa Seed Extracts on Intestinal Barrier and Inflammation in a Co-Culture System of an Enterohemorrhagic Escherichia coli and Human Intestinal Cells
IF: 4.546 -
Sodium Tanshinone II Sulfonate A Ameliorates Hypoxia-Induced Pulmonary Hypertension
IF: 4.225Journal： Frontiers in Pharmacology (2020)PMID：32508639Reactivity：RatSample Type：Tissue homogenate -
NRF2 -617 C/A Polymorphism Impacts Proinflammatory Cytokine Levels, Survival, and Transplant-Related Mortality After Hematopoietic Stem Cell Transplantation in Adult Patients Receiving Busulfan-Based Conditioning Regimens
IF: 4.225
实验操作视频
