Human IL-8(Interleukin 8) ELISA Kit
中文名称：人白介素8(IL-8)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上，实验时样品（或标准品）中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素，抗人IL-8抗体与结合在包被抗体上的人IL-8结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-8浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-8的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-8，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Isolation, characterization and analysis of pro-inflammatory potential of Klebsiella pneumoniae outer membrane vesicles
IF: 2.581Journal： MICROBIAL PATHOGENESIS (2019)PMID：31493501Reactivity：HumanSample Type：Cell culture supernatant -
Association of IL-6, IL-8, MMP-13 gene polymorphisms with knee osteoarthritis susceptibility in the Chinese Han population
IF: 2.535 -
Functional characterisation of Holothuria leucospilota Fas-associated death domain in the innate immune-related signalling pathways.
IF: 2.173 -
Leptin induces IL-6 and IL-8 expression through leptin receptor Ob-Rb in human dental pulp fibroblasts
IF: 1.565Journal： ACTA ODONTOLOGICA SCANDINAVICA (2019)PMID：30600742Reactivity：HumanSample Type：Cell culture supernatant -
Interleukin 8 as predictive factor for response to chemotherapy in colorectal cancer patients
IF: 0.960 -
The enhancer RNA lnc-SLC4A1-1 epigenetically regulates unexplained recurrent pregnancy loss (URPL) by activating CXCL8 and NF-kB pathway
IF: 6.183 -
Associations between knee structural measures, circulating inflammatory factors and MMP13 in patients with knee osteoarthritis
IF: 5.454 -
Impact of an Early Oral Feeding Protocol on Inflammatory Cytokine Changes After Esophagectomy
IF: 3.780Journal： ANNALS OF THORACIC SURGERY (2018)Reactivity：HumanSample Type：serum -
Probiotic attributes and prevention of LPS-induced pro-inflammatory stress in RAW264.7 macrophages and human intestinal epithelial cell line (Caco-2) by newly isolated Weissella cibaria strains
IF: 3.289 -
RETRACTED: LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203
IF: 3.234
实验操作视频
