Human IL-8(Interleukin 8) ELISA Kit
中文名称：人白介素8(IL-8)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人IL-8抗体包被于酶标板上，实验时样品（或标准品）中的人IL-8会与包被抗体结合。后依次加入生物素化的抗人IL-8抗体和辣根过氧化物酶标记的亲和素，抗人IL-8抗体与结合在包被抗体上的人IL-8结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，IL-8浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中IL-8的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人IL-8，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Validation of Salivary Markers, IL-1β, IL-8 and Lgals3bp for Detection of Oral Squamous Cell Carcinoma in an Indian Population
IF: 3.998 -
Mammalian Ste20-like kinase 4 inhibits the inflammatory response in Aspergillus fumigatus keratitis
IF: 3.943Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2020)PMID：33182037Reactivity：HumanSample Type：Cell lysate -
Nec-1 Attenuates Neurotoxicity Induced by Titanium Dioxide Nanomaterials on Sh-Sy5y Cells Through RIP1
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Breast cancer cells promote self-migration by secreting interleukin 8 to induce NET formation
IF: 2.984Journal： GENE (2020)Reactivity：HumanSample Type：normal breast tissue,breast tumor tissue -
Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF?κB activation
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The clinical value of interleukins-8, -10, and -17 in idiopathic granulomatous mastitis
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A case of asthma with Behcet’s disease: successful treatment with omalizumab and its effects on recurrent aphthous lesions
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Nec-1 attenuates inflammation and cytotoxicity induced by high glucose on THP-1 derived macrophages through RIP1
IF: 1.931Journal： ARCHIVES OF ORAL BIOLOGY (2020)PMID：32805637Reactivity：HumanSample Type：Cell culture supernatant -
Activation of NF-κB pathways mediating the inflammation and pulmonary diseases associated with atmospheric methylamine exposure
IF: 5.714Journal： ENVIRONMENTAL POLLUTION (2019)PMID：31252119Reactivity：HumanSample Type：Cell culture supernatant -
Inhibition of CREB-mediated ZO-1 and activation of NF-κB-induced IL-6 by colonic epithelial MCT4 destroys intestinal barrier function
IF: 5.039
实验操作视频
