Human INS(Insulin) ELISA Kit
中文名称：人胰岛素(INS)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 μIU/mL
- 灵敏度： 0.47 μIU/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人INS抗体包被于酶标板上，实验时样品（或标准品）中的人INS会与包被抗体结合。后依次加入生物素化的抗人INS抗体和辣根过氧化物酶标记的亲和素，抗人INS抗体与结合在包被抗体上的人INS结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，INS浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中INS的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 μIU/mL |
| 灵敏度 | 0.47 μIU/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人INS，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Effect of saccharin, a non-nutritive sweeteners, on insulin and blood glucose levels in healthy young men: A crossover trial
Journal： Diabetes & Metabolic Syndrome-Clinical Research & Reviews (2022)PMID：35598544Reactivity：HumanSample Type：plasma -
The association of LEPR Q223R polymorphism with type 2 diabetes mellitus in Malaysia
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The Impact of Metabolic Syndrome on Quality of Life Among Individuals With Knee Osteoarthritis Living in Egypt.
Journal： Clinical Medicine Insights-Arthritis and Musculoskeletal Disorders (2022)Reactivity：HumanSample Type：serum -
Cardiovascular Disease Prediction by Machine Learning Algorithms Based on Cytokines in Kazakhs of China
IF: 4.790 -
Prevalence of ApoB100 rs693 gene polymorphism in metabolic syndrome among female students at King Abdulaziz University
IF: 4.219Journal： SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021)PMID：34121862Reactivity：HumanSample Type：Serum -
Acute and short-term effects of Lactobacillus paracasei subsp. paracasei 431 and inulin intake on appetite control and dietary intake: A two-phases randomized, double blind, placebo-controlled study
IF: 3.868 -
The effect of hydrothermal processing on the performance of broiler chicks fed corn or sorghum-based diets
IF: 3.247Journal： ANIMAL FEED SCIENCE AND TECHNOLOGY (2021)Reactivity：ChickenSample Type：Serum -
Fermented soybean enhances post-meal response in appetite-regulating hormones among Indonesian girls with obesity
IF: 2.288Journal： Obesity Research & Clinical Practice (2021)PMID：34147377Reactivity：HumanSample Type：plasma -
Effect of Lycium barbarum polysaccharide supplementation in non-alcoholic fatty liver disease patients: study protocol for a randomized controlled trial
IF: 2.279 -
Association of Apelin gene rs3115757 variation and expression with metabolic syndrome among female students in King Abdulaziz University
实验操作视频
