Human KIM-1(Kidney Injury Molecule 1) ELISA Kit
中文名称：人肾损伤分子1(KIM-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人KIM-1抗体包被于酶标板上，实验时样品（或标准品）中的人KIM-1会与包被抗体结合。后依次加入生物素化的抗人KIM-1抗体和辣根过氧化物酶标记的亲和素，抗人KIM-1抗体与结合在包被抗体上的人KIM-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，KIM-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中KIM-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人KIM-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Association of acute exposure to PM2.5 constituents and sources with kidney injury: A longitudinal panel study of Nrf2 promoter polymorphism
IF: 12.200Journal： JOURNAL OF HAZARDOUS MATERIALS (2024)Reactivity：HumanSample Type：plasma -
Serum high mobility group box 1 as a potential biomarker for the progression of kidney disease in patients with type 2 diabetes
IF: 7.300 -
Unveiling the role of mtDNA in Liver-Kidney Crosstalk: Insights from trichloroethylene hypersensitivity syndrome
IF: 4.800Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2024)Reactivity：HumanSample Type：Serum -
Preparation and characterization of cysteine-rich collagen peptide and its antagonistic effect on microplastic induced damage to HK-2?cells
IF: 4.800 -
The Relationship between Circulating Kidney Injury Molecule-1 and Cardiovascular Morbidity and Mortality in Hemodialysis Patients
IF: 3.900 -
Diagnostic and Prognostic Values of KLF5 and RUNX1 in Acute Kidney Injury in Septic Patients
IF: 3.100Journal： APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2024)Reactivity：HumanSample Type：Serum -
The Use of Kidney Biomarkers, Nephrin and KIM-1, for the Detection of Early Glomerular and Tubular Damage in Patients with Acromegaly: A Case–Control Pilot Study
IF: 2.900 -
Tubular biomarkers and histology in lupus nephritis
IF: 2.400Journal： International Journal of Rheumatic Diseases (2024)Reactivity：HumanSample Type：Serum -
Comparison of Renal Tubular Damage with Kidney Injury Molecule-1 in Open and Laparoscopic Colorectal Cancer Surgery
IF: 2.400 -
Effect of access sheath diameter used in percutaneous nephrolithotomy on renal function: a prospective randomized study
IF: 2.000Journal： Urolithiasis (2024)Reactivity：HumanSample Type：Urine sample
实验操作视频
