Human LDHA(Lactate Dehydrogenase A) ELISA Kit
中文名称：人乳酸脱氢酶A(LDHA)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 0.78-50 ng/mL
- 灵敏度： 0.47 ng/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人LDHA抗体包被于酶标板上，实验时样品（或标准品）中的人LDHA会与包被抗体结合。后依次加入生物素化的抗人LDHA抗体和辣根过氧化物酶标记的亲和素，抗人LDHA抗体与结合在包被抗体上的人LDHA结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LDHA浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LDHA的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 0.78-50 ng/mL |
| 灵敏度 | 0.47 ng/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人LDHA，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
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Evaluation of Serum Pyruvate Kinase M2 and Key Glycolytic Signatures and Its Correlation with Phosphatidylinositol-3-kinase/Akt/Mammalian Target of Rapamycin Pathway in Women with Breast Carcinoma
IF: 1.000Journal： Biomedical and Biotechnology Research Journal (2024)Reactivity：HumanSample Type：Serum -
A Proposed Association between Improving Energy Metabolism of HepG2 Cells by Plant Extracts and Increasing Their Sensitivity to Doxorubicin
IF: 4.600 -
Stress granules inhibit endoplasmic reticulum stress-mediated apoptosis during hypoxia-induced injury in acute liver failure
IF: 4.300 -
Evaluation of mutagenic, cytotoxic, mitochondrial dysfunction, apoptotic activity, and acute toxicity of ethanolic extract of Cissus quadrangularis
IF: 4.571 -
The effect of coenzyme Q10 plus trimetazidine on acute viral myocarditis treatment
IF: 4.060Journal： American Journal of Translational Research (2021)DOI：PMID:35035725PMID：35035725Reactivity：HumanSample Type：serum -
Combination of oncolytic adenovirus ZD55 harboring TRAIL-IETD-MnSOD and cytokine-induced killer cells against lung cancer
IF: 3.932Journal： Annals of Translational Medicine (2021)PMID：34790733Reactivity：Human,MouseSample Type：serum -
MiR-181a-5p facilitates proliferation, invasion, and glycolysis of breast cancer through NDRG2-mediated activation of PTEN/AKT pathway
IF: 3.269 -
Investigation of glucose catabolism in hypoxic Mcf 7 breast cancer culture
IF: 2.058 -
Inhibition of glycolysis by targeting lactate dehydrogenase A facilitates hyaluronan synthase 2 synthesis in synovial fibroblasts of temporomandibular joint osteoarthritis
IF: 4.147 -
Downregulation of P300/CBP-Associated Factor Attenuates Myocardial Ischemia-Reperfusion Injury Via Inhibiting Autophagy
IF: 2.523Journal： International Journal of Medical Sciences (2020)PMID：32547315Reactivity：HumanSample Type：Serum,Cell culture supernatant
Q1:我想问问有没有肝功能检测方面的ELISA试剂盒
肝功能相关有以下几种指标，供您参考
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