Human LEP(Leptin) ELISA Kit
中文名称：人瘦素(LEP)酶联免疫吸附测定试剂盒
别称：
Price:
Size:
96 T48 T96 T × 5
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人LEP抗体包被于酶标板上，实验时样品（或标准品）中的人LEP会与包被抗体结合。后依次加入生物素化的抗人LEP抗体和辣根过氧化物酶标记的亲和素，抗人LEP抗体与结合在包被抗体上的人LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 | 96 T/ 48 T/ 96 T × 5 |
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
Leptin-mediated suppression of lipoprotein lipase cleavage enhances lipid uptake and facilitates lymph node metastasis in gastric cancer
IF: 20.100Lipid metabolic reprogramming mediated by circulating Nrg4 alleviates metabolic dysfunction-associated steatotic liver disease during the early recovery phase after sleeve gastrectomy
IF: 7.000Role of Adipose Tissue Hormones in Pathogenesis of Cryptoglandular Anal Fistula
IF: 5.600Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)PMID：38338780Reactivity：HumanSample Type：cryptoglandular fistulasHighly Concentrated Stabilized Hybrid Complexes of Hyaluronic Acid: Rheological and Biological Assessment of Compatibility with Adipose Tissue and Derived Stromal Cells towards Regenerative Medicine
IF: 5.600Exploring the Link Between Psoriasis and Adipose Tissue: One Amplifies the Other
IF: 4.900Journal： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2024)Reactivity：HumanSample Type：Adipose tissue (AT)Composition of gut microbiota in obese and normal-weight Uygur adults and its association with adipocyte-related factors
IF: 3.800Correlation of Adiponectin and Leptin with Anthropometrics and Behavioral and Physical Performance in Overweight and Obese Chinese College Students
IF: 3.600Adipocytes Promote Endometrial Cancer Progression Through Activation of the SIRT1-HMMR Signaling Axis
IF: 3.000White Tea Consumption Alleviates Anthropometric and Metabolic Parameters in Obese Patients
IF: 2.400The role of adipokines and ghrelin in interactions and clinical implications in childhood obesity
IF: 1.400Journal： Journal of Education and Health Promotion (2024)PMID：38545313Reactivity：HumanSample Type：serum
Q1:循环瘦素和瘦素是一个指标吗？
循环瘦素就是指瘦素，循环是形容瘦素水平的循环波动
