Human LEP(Leptin) ELISA Kit
中文名称：人瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人LEP抗体包被于酶标板上，实验时样品（或标准品）中的人LEP会与包被抗体结合。后依次加入生物素化的抗人LEP抗体和辣根过氧化物酶标记的亲和素，抗人LEP抗体与结合在包被抗体上的人LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Ramadan Intermittent Fasting Is Associated with Changes in Circulating Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) in Metabolically Healthy Obese Subjects
IF: 2.948 -
The protective effect of N-acetyl cysteine against selenium toxicity and gamma irradiation in rats
IF: 2.597 -
The association of LEPR Q223R polymorphism with type 2 diabetes mellitus in Malaysia
-
Leptin correlates with monocytes activation and severe condition in COVID-19 patients
IF: 4.962 -
Obesity-related proteins score as a potential marker of breast cancer risk
IF: 4.380 -
Body composition, adipokines, FGF23-Klotho and bone in kidney transplantation: Is there a link?
IF: 3.902 -
New insights into the metabolic-bone crosstalk in active acromegaly
IF: 1.582 -
Obesity and Breast Cancer: Association of Serum Adiponectin, Leptin, and Adiponectin–Leptin Ratio as Risk Biomarkers
Journal： Indian Journal of Medical and Paediatric Oncology (2021)Reactivity：HumanSample Type：Serum -
Association of rs699947 (?2578 C/A) and rs2010963 (?634 G/C) Single Nucleotide Polymorphisms of the VEGF Gene, VEGF-A and Leptin Serum Level, and Cardiovascular Risk in Patients with Excess Body Mass: A Case–Control Study
IF: 3.303 -
Circadian rhythm abnormalities in patients with inflammatory bowel disease – association with adipokine profile
IF: 2.130Journal： SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY (2020)PMID：32180483Reactivity：HumanSample Type：Serum
实验操作视频
