Human LEP(Leptin) ELISA Kit
中文名称：人瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人LEP抗体包被于酶标板上，实验时样品（或标准品）中的人LEP会与包被抗体结合。后依次加入生物素化的抗人LEP抗体和辣根过氧化物酶标记的亲和素，抗人LEP抗体与结合在包被抗体上的人LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Effect of laparoscopic sleeve gastrectomy on renal function in obese patients
IF: 1.355 -
Serum adipocytokines and adiposity as predictive indices of preeclampsia
-
Porous graphene-black phosphorus nanocomposite modified electrode for detection of leptin
IF: 9.518 -
Contrasting short-term temperature effects on the profiling of metabolic and stress hormones in non-obese healthy adults: A randomized cross-over trial
IF: 5.026 -
Adiponectin Receptors and Pro-inflammatory Cytokines Are Modulated in Common Variable Immunodeficiency Patients: Correlation With Ig Replacement Therapy
IF: 4.716 -
Adiponectin Expression Is Modulated by Long-Term Physical Activity in Adult Patients Affected by Cystic Fibrosis
IF: 3.545 -
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals
IF: 3.239Journal： DIABETES RESEARCH AND CLINICAL PRACTICE (2019)PMID：31150725Reactivity：HumanSample Type：Serum -
Leptin, resistin and visfatin as useful predictors of gestational diabetes mellitus
IF: 2.651 -
High Ghrelin Level Predicts the Curve Progression of Adolescent Idiopathic Scoliosis Girls
IF: 2.583 -
Elevated leptin levels in temporomandibular joint osteoarthritis promote proinflammatory cytokine IL-6 expression in synovial fibroblasts
IF: 2.237Journal： JOURNAL OF ORAL PATHOLOGY & MEDICINE (2018)PMID：30597628Reactivity：HumanSample Type：Cell culture supernatant
实验操作视频
