Human LEP(Leptin) ELISA Kit
中文名称：人瘦素(LEP)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 15.63-1000 pg/mL
- 灵敏度： 9.38 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人LEP抗体包被于酶标板上，实验时样品（或标准品）中的人LEP会与包被抗体结合。后依次加入生物素化的抗人LEP抗体和辣根过氧化物酶标记的亲和素，抗人LEP抗体与结合在包被抗体上的人LEP结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，LEP浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中LEP的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 15.63-1000 pg/mL |
| 灵敏度 | 9.38 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人LEP，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Adiponectin Expression Is Modulated by Long-Term Physical Activity in Adult Patients Affected by Cystic Fibrosis
IF: 3.545 -
Effect of Ramadan diurnal fasting on visceral adiposity and serum adipokines in overweight and obese individuals
IF: 3.239Journal： DIABETES RESEARCH AND CLINICAL PRACTICE (2019)PMID：31150725Reactivity：HumanSample Type：Serum -
Leptin, resistin and visfatin as useful predictors of gestational diabetes mellitus
IF: 2.651 -
Fasting serum leptin levels in elderly women with major depressive disorder: a case-control study.
IF: 0.698 -
High Ghrelin Level Predicts the Curve Progression of Adolescent Idiopathic Scoliosis Girls
IF: 2.583 -
Elevated leptin levels in temporomandibular joint osteoarthritis promote proinflammatory cytokine IL-6 expression in synovial fibroblasts
IF: 2.237Journal： JOURNAL OF ORAL PATHOLOGY & MEDICINE (2018)PMID：30597628Reactivity：HumanSample Type：Cell culture supernatant -
Roles and mechanisms of leptin in osteogenic stimulation in cervical ossification of the posterior longitudinal ligament
IF: 1.610Journal： Journal of Orthopaedic Surgery and Research (2018)PMID：29970120Reactivity：HumanSample Type：Serum -
Correlation of insulin?resistance with blood fat and glucose in elder patients after surgery for hepatic carcinoma
IF: 1.410Journal： Experimental and Therapeutic Medicine (2018)PMID：30651864Reactivity：HumanSample Type：Serum -
Placental peptides metabolism and maternal factors as predictors of risk of gestational diabetes in pregnant women. A case-control study
IF: 2.806 -
LEPR gene polymorphism and plasma soluble leptin receptor levels are associated with polycystic ovary syndrome in Han Chinese women
IF: 1.010
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