Human LH(Luteinizing Hormone) ELISA Kit
中文名称:人促黄体生成激素(LH)酶联免疫吸附测定试剂盒
别称:

Price:
Size:
96 T48 T96 T × 5
- 反应性: Human
- 检测范围: 0.16-10 mIU/mL
- 灵敏度: 0.1 mIU/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人LH抗体包被于酶标板上,实验时样品(或标准品)中的人LH会与包被抗体结合。后依次加入生物素化的抗人LH抗体和辣根过氧化物酶标记的亲和素,抗人LH抗体与结合在包被抗体上的人LH结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,LH浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中LH的浓度。 |
反应类型 | Sandwich-ELISA |
规格 | 96 T/ 48 T/ 96 T × 5 |
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 0.16-10 mIU/mL |
灵敏度 | 0.1 mIU/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人LH,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
Assessment Between Follicular Fluid Multiple Element Concentrations and Hormonal Profiles of Women with Polycystic Ovary Syndrome
IF: 6.700Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway
IF: 7.666The impact of dolutegravir-based combination antiretroviral therapy on the spermatozoa and fertility parameters of men living with human immunodeficiency virus
IF: 2.532Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K-Akt pathway
IF: 3.906Pueratin improves diminished ovarian reserve by inhibiting apoptosis
IF: 2.447High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies
IF: 3.585Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY (2019)PMID:30641075Reactivity:HumanSample Type:Serum,Cell culture mediumEffect of gestational exposure to arsenic on puberty in offspring female mice
IF: 4.427ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats
IF: 5.965