Human LL-37(Antibacterial Protein LL-37) ELISA Kit
中文名称:人杀菌肽LL-37(LL-37)酶联免疫吸附测定试剂盒
别称:

Price:
Size:
96 T48 T96 T × 5
- 反应性: Human
- 检测范围: 1.56-100 ng/mL
- 灵敏度: 0.94 ng/mL
产品应用 | ELISA |
检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人LL-37抗体包被于酶标板上,实验时样品(或标准品)中的人LL-37会与包被抗体结合。后依次加入生物素化的抗人LL-37抗体和辣根过氧化物酶标记的亲和素,抗人LL-37抗体与结合在包被抗体上的人LL-37结合,生物素与亲和素特异性结合而形成免疫复合物,游离的成分被洗去。加入显色底物(TMB),TMB在辣根过氧化物酶的催化下呈现蓝色,加终止液后变成黄色。用酶标仪在450 nm波长处测OD值,LL-37浓度与OD450值之间呈正比,通过绘制标准曲线计算出样品中LL-37的浓度。 |
反应类型 | Sandwich-ELISA |
规格 | 96 T/ 48 T/ 96 T × 5 |
反应时间 | 3 h 30 min |
反应性 | Human |
检测方法 | 比色法;ELISA;夹心法 |
检测范围 | 1.56-100 ng/mL |
灵敏度 | 0.94 ng/mL |
样本体积 | 100 μL |
样本类型 | 血清、血浆或其他生物体液 |
特异性 | 可检测样本中的人LL-37,且与其它类似物无明显交叉反应 |
精密度 | 板内,板间变异系数均<10% |
回收率 | 80%-120% |
储存条件 | 2-8℃ |
有效期 | 12个月 |
数据处理 |
Antibacterial periodontal ligament stem cells enhance periodontal regeneration and regulate the oral microbiome
IF: 7.100Modulation of innate immunity related genes resulting in prophylactic antimicrobial and antiviral properties
IF: 6.100Antimicrobial peptide LL37 and regulatory T cell associated with late-onset sepsis in very preterm infants
IF: 4.600The Utility of Noninvasive Urinary Biomarkers for the Evaluation of Vesicoureteral Reflux in Children
IF: 5.600Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023)PMID:38139407Reactivity:HumanSample Type:urineAssociations of Serum Antimicrobial Peptide LL-37 with Longitudinal Cognitive Decline and Neurodegeneration Among Older Adults with Memory Complaints
IF: 4.000Cord blood antimicrobial peptide LL37 levels in preterm neonates and association with preterm complications
IF: 3.288Rapid and Effective Vitamin D Supplementation May Present Better Clinical Outcomes in COVID-19 (SARS-CoV-2) Patients by Altering Serum INOS1, IL1B, IFNg, Cathelicidin-LL37, and ICAM1
IF: 5.719Prediction of Treatment Outcome with Inflammatory Biomarkers after 2 Months of Therapy in Pulmonary Tuberculosis Patients: Preliminary Results
IF: 3.492Serum and cerebrospinal fluid host proteins indicate stroke in children with tuberculous meningitis
IF: 3.240Candidalysin Is a Potent Trigger of Alarmin and Antimicrobial Peptide Release in Epithelial Cells
IF: 4.366