Human MAPτ(Microtubule Associated Protein Tau/Tau Protein) ELISA Kit
中文名称：人微管关联蛋白(MAPτ)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 7.81-500 pg/mL
- 灵敏度： 4.69 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MAPτ抗体包被于酶标板上，实验时样品（或标准品）中的人MAPτ会与包被抗体结合。后依次加入生物素化的抗人MAPτ抗体和辣根过氧化物酶标记的亲和素，抗人MAPτ抗体与结合在包被抗体上的人MAPτ结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MAPτ浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MAPτ的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 7.81-500 pg/mL |
| 灵敏度 | 4.69 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MAPτ，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Amyloid and Tau as cerebrospinal fluid biomarkers in anti-N-Methyl-D-aspartate receptor encephalitis
IF: 3.300Journal： NEUROLOGICAL SCIENCES (2024)PMID：38280087Reactivity：HumanSample Type：cerebrospinal fluid (CSF) -
Unraveling the neuroprotective mechanisms of naltrexone against aluminum-induced neurotoxicity
IF: 2.600 -
Walnut oil: a promising nutraceutical in reducing oxidative stress and improving cholinergic activity in an in vitro Alzheimer’s disease model
IF: 2.200 -
Clemastine fumarate attenuates tauopathy and meliorates cognition in hTau mice via autophagy enhancement
IF: 5.600Journal： INTERNATIONAL IMMUNOPHARMACOLOGY (2023)PMID：37494840Reactivity：MouseSample Type：hippocampal tissue -
Plasma Aβ level alterations after sleep deprivation correspond to brain structural remodeling in medical night shift workers
IF: 3.800 -
Evaluation of the effects of herpes simplex glycoprotein B on complement system and cytokines in in vitro models of Alzheimer's disease
IF: 3.300Journal： JOURNAL OF APPLIED TOXICOLOGY (2023)DOI：10.1002/jat.4471PMID：36999203Reactivity：HumanSample Type：SH-SY5Y cell -
Antioxidant dihydrolipolic acid protects against in vitro aluminum-induced toxicity
IF: 3.300 -
Evaluation of possible neuroprotective effects of virgin coconut oil on aluminum-induced neurotoxicity in an in vitro Alzheimer's disease model
IF: 3.300 -
Serum tau protein and myelin basic protein in pediatric patients with congenital heart defects undergoing cardiac surgery: preliminary assessment as novel neuromarkers of brain injury
IF: 2.100 -
Modeling the early stages of Alzheimer’s disease by administering intracerebroventricular injections of human native Aβ oligomers to rats
IF: 7.578Journal： Acta Neuropathologica Communications (2022)PMID：35974377Reactivity：HumanSample Type：brain
实验操作视频
