Human MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit
中文名称：人单核细胞趋化蛋白1(MCP-1)酶联免疫吸附测定试剂盒
Price:
- 反应性： Human
- 检测范围： 62.5-4000 pg/mL
- 灵敏度： 37.5 pg/mL
| 产品应用 | ELISA |
| 检测原理 | 本试剂盒采用双抗体夹心ELISA法。用抗人MCP-1抗体包被于酶标板上，实验时样品（或标准品）中的人MCP-1会与包被抗体结合。后依次加入生物素化的抗人MCP-1抗体和辣根过氧化物酶标记的亲和素，抗人MCP-1抗体与结合在包被抗体上的人MCP-1结合，生物素与亲和素特异性结合而形成免疫复合物，游离的成分被洗去。加入显色底物(TMB)，TMB在辣根过氧化物酶的催化下呈现蓝色，加终止液后变成黄色。用酶标仪在450 nm波长处测OD值，MCP-1浓度与OD450值之间呈正比，通过绘制标准曲线计算出样品中MCP-1的浓度。 |
| 反应类型 | Sandwich-ELISA |
| 规格 |
96 T
/ 48 T
/ 96 T × 5
|
| 反应时间 | 3 h 30 min |
| 反应性 | Human |
| 检测方法 | 比色法;ELISA;夹心法 |
| 检测范围 | 62.5-4000 pg/mL |
| 灵敏度 | 37.5 pg/mL |
| 样本体积 | 100 μL |
| 样本类型 | 血清、血浆或其他生物体液 |
| 特异性 | 可检测样本中的人MCP-1，且与其它类似物无明显交叉反应 |
| 精密度 | 板内，板间变异系数均<10% |
| 回收率 | 80%-120% |
| 储存条件 | 2-8℃ |
| 有效期 | 12个月 |
| 数据处理 |
-
Breast cancer-derived CAV1 promotes lung metastasis by regulating integrin α6β4 and the recruitment and polarization of tumor-associated neutrophils
IF: 8.200Journal： International Journal of Biological Sciences (2024)Reactivity：Human -
Molecular and immunological features of TREM1 and its emergence as a prognostic indicator in glioma
IF: 7.300 -
N-acetyltransferase 10 affects the proliferation of intrahepatic cholangiocarcinoma and M2-type polarization of macrophages by regulating C-C motif chemokine ligand 2
IF: 6.100 -
Anti-atherogenic mechanism of ethanol extract of Christia vespertilionis (L.f.) Bakh. F. Leaves in vitro
IF: 4.800 -
Preparation and characterization of cysteine-rich collagen peptide and its antagonistic effect on microplastic induced damage to HK-2?cells
IF: 4.800 -
Pirfenidone inhibits CCL2-mediated Treg chemotaxis induced by palbociclib and fulvestrant in HR+/HER2? breast cancer
IF: 4.800 -
Atractylodin modulates ASAH3L to improve galactose metabolism and inflammation to alleviate acute lung injury
IF: 4.600 -
Exacerbation of atherosclerosis by STX17 knockdown: Unravelling the role of autophagy and inflammation
IF: 4.300 -
Ginsenoside Rb3 alleviates the formation of osteoclasts induced by periodontal ligament fibroblasts in the periodontitis microenvironment through the STAT3 pathway
IF: 3.300 -
Amphotericin B for injection triggers degranulation of human LAD2 mast cells by MRGPRX2 and pseudo-allergic reactions in mice via MRGPRB2 activation
IF: 3.300
实验操作视频
